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Alterations of new methylated phospholipid synthesis in the plasma membranes of macrophages exposed to chemoattractants.

Publication ,  Journal Article
Pike, MC; Snyderman, R
Published in: J Cell Biol
October 1981

Chemotactic factors have been shown to inhibit the methylation of phosphatidylethanolamine in macrophages without affecting total phospholipid synthesis. It would thus be anticipated that newly synthesized membranes of macrophages exposed to chemoattractants would have an increased ratio of phosphatidylethanolamine to its methylated derivatives. These ratios were measured directly in newly synthesized phospholipids of plasma membranes isolated from guinea pig peritoneal macrophages. The phosphatidylethanolamine: methylated phospholipid ratio in such plasma membranes was increased by 53 to 111% upon exposure of the cells to chemotactic factors. This increase was due to decreased synthesis of methylated phospholipids and not to altered formation of phosphatidylethanolamine or activation of phospholipases. Methylated phospholipid ratios were also studied in the leading front lamellipodia isolated from macrophages migrating under chemotactic and nonchemotactic conditions. The phosphatidylethanolamine:methylated phospholipid ratios were increased up to fourfold in lamellipodia of macrophages migrating towards chemotactic agents when compared to those from cells migrating randomly. Biophysical changes in the plasma membrane produced by an increase in the ratio of phosphatidylethanolamine:methylated phospholipids as a result of exposure of cells to chemoattractants may be required for sustained directed migration.

Duke Scholars

Published In

J Cell Biol

DOI

ISSN

0021-9525

Publication Date

October 1981

Volume

91

Issue

1

Start / End Page

221 / 226

Location

United States

Related Subject Headings

  • Phosphatidylethanolamines
  • Phosphatidylcholines
  • Oligopeptides
  • N-Formylmethionine Leucyl-Phenylalanine
  • N-Formylmethionine
  • Methylation
  • Membrane Lipids
  • Macrophages
  • Guinea Pigs
  • Developmental Biology
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Pike, M. C., & Snyderman, R. (1981). Alterations of new methylated phospholipid synthesis in the plasma membranes of macrophages exposed to chemoattractants. J Cell Biol, 91(1), 221–226. https://doi.org/10.1083/jcb.91.1.221
Pike, M. C., and R. Snyderman. “Alterations of new methylated phospholipid synthesis in the plasma membranes of macrophages exposed to chemoattractants.J Cell Biol 91, no. 1 (October 1981): 221–26. https://doi.org/10.1083/jcb.91.1.221.
Pike, M. C., and R. Snyderman. “Alterations of new methylated phospholipid synthesis in the plasma membranes of macrophages exposed to chemoattractants.J Cell Biol, vol. 91, no. 1, Oct. 1981, pp. 221–26. Pubmed, doi:10.1083/jcb.91.1.221.

Published In

J Cell Biol

DOI

ISSN

0021-9525

Publication Date

October 1981

Volume

91

Issue

1

Start / End Page

221 / 226

Location

United States

Related Subject Headings

  • Phosphatidylethanolamines
  • Phosphatidylcholines
  • Oligopeptides
  • N-Formylmethionine Leucyl-Phenylalanine
  • N-Formylmethionine
  • Methylation
  • Membrane Lipids
  • Macrophages
  • Guinea Pigs
  • Developmental Biology