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Inhibitors of metalloendoproteases block spiculogenesis in sea urchin primary mesenchyme cells.

Publication ,  Journal Article
Roe, JL; Park, HR; Strittmatter, WJ; Lennarz, WJ
Published in: Exp Cell Res
April 1989

Metalloendoproteases have been implicated in a variety of fusion processes including plasma membrane fusion and exocytosis. As a prerequisite to skeleton formation in the sea urchin embryo, primary mesenchyme cells undergo fusion via filopodia to form syncytia. The spicule is formed within the syncytial cable by matrix and mineral deposition. To investigate the potential involvement of a metalloendoprotease in spiculogenesis, the effect of inhibitors of this enzyme on skeleton formation was studied. Experiments with primary mesenchyme cells in vitro and in normal embryos revealed that skeleton formation was blocked by these inhibitors. These findings implicate a metalloendoprotease in spiculogenesis; such an enzyme has been demonstrated in homogenates of primary mesenchyme cells. The most likely site of action of the metalloendoprotease is at the cell membrane fusion stage and/or at subsequent events requiring membrane fusion.

Duke Scholars

Published In

Exp Cell Res

DOI

ISSN

0014-4827

Publication Date

April 1989

Volume

181

Issue

2

Start / End Page

542 / 550

Location

United States

Related Subject Headings

  • Sea Urchins
  • Pseudopodia
  • Phenanthrolines
  • Metalloendopeptidases
  • Mesoderm
  • Membrane Fusion
  • Gastrula
  • Dipeptides
  • Cells, Cultured
  • Cell Differentiation
 

Citation

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Roe, J. L., Park, H. R., Strittmatter, W. J., & Lennarz, W. J. (1989). Inhibitors of metalloendoproteases block spiculogenesis in sea urchin primary mesenchyme cells. Exp Cell Res, 181(2), 542–550. https://doi.org/10.1016/0014-4827(89)90110-9
Roe, J. L., H. R. Park, W. J. Strittmatter, and W. J. Lennarz. “Inhibitors of metalloendoproteases block spiculogenesis in sea urchin primary mesenchyme cells.Exp Cell Res 181, no. 2 (April 1989): 542–50. https://doi.org/10.1016/0014-4827(89)90110-9.
Roe JL, Park HR, Strittmatter WJ, Lennarz WJ. Inhibitors of metalloendoproteases block spiculogenesis in sea urchin primary mesenchyme cells. Exp Cell Res. 1989 Apr;181(2):542–50.
Roe, J. L., et al. “Inhibitors of metalloendoproteases block spiculogenesis in sea urchin primary mesenchyme cells.Exp Cell Res, vol. 181, no. 2, Apr. 1989, pp. 542–50. Pubmed, doi:10.1016/0014-4827(89)90110-9.
Roe JL, Park HR, Strittmatter WJ, Lennarz WJ. Inhibitors of metalloendoproteases block spiculogenesis in sea urchin primary mesenchyme cells. Exp Cell Res. 1989 Apr;181(2):542–550.
Journal cover image

Published In

Exp Cell Res

DOI

ISSN

0014-4827

Publication Date

April 1989

Volume

181

Issue

2

Start / End Page

542 / 550

Location

United States

Related Subject Headings

  • Sea Urchins
  • Pseudopodia
  • Phenanthrolines
  • Metalloendopeptidases
  • Mesoderm
  • Membrane Fusion
  • Gastrula
  • Dipeptides
  • Cells, Cultured
  • Cell Differentiation