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Requirement for metalloendoprotease in exocytosis: evidence in mast cells and adrenal chromaffin cells.

Publication ,  Journal Article
Mundy, DI; Strittmatter, WJ
Published in: Cell
March 1985

Exocytosis is initiated by the receptor-mediated influx of calcium that results in fusion of the secretory vesicle with the plasma membrane. We examined the possibility that calcium-dependent exocytosis in mast cells and adrenal chromaffin cells requires metalloendoprotease activity. Metalloendoprotease inhibitors and dipeptide substrates block exocytosis in these cells with the same specificity and dose dependency as that with which they interact with metalloendoproteases. Metalloendoprotease activity is identified in these cells with fluorogenic synthetic substrates, which also blocked exocytosis. Metalloendoprotease activity is highest in the plasma membrane of chromaffin cells. The metalloendoprotease appears to be required in exocytosis at a step dependent on or after calcium entry, since exocytosis initiated by direct calcium introduction in both mast cells and chromaffin cells is blocked by metalloendoprotease inhibitors.

Duke Scholars

Published In

Cell

DOI

ISSN

0092-8674

Publication Date

March 1985

Volume

40

Issue

3

Start / End Page

645 / 656

Location

United States

Related Subject Headings

  • Rats, Inbred Strains
  • Rats
  • Protease Inhibitors
  • Phenanthrolines
  • Oligopeptides
  • Metalloendopeptidases
  • Mast Cells
  • Histamine Release
  • Female
  • Exocytosis
 

Citation

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Mundy, D. I., & Strittmatter, W. J. (1985). Requirement for metalloendoprotease in exocytosis: evidence in mast cells and adrenal chromaffin cells. Cell, 40(3), 645–656. https://doi.org/10.1016/0092-8674(85)90213-2
Mundy, D. I., and W. J. Strittmatter. “Requirement for metalloendoprotease in exocytosis: evidence in mast cells and adrenal chromaffin cells.Cell 40, no. 3 (March 1985): 645–56. https://doi.org/10.1016/0092-8674(85)90213-2.
Mundy, D. I., and W. J. Strittmatter. “Requirement for metalloendoprotease in exocytosis: evidence in mast cells and adrenal chromaffin cells.Cell, vol. 40, no. 3, Mar. 1985, pp. 645–56. Pubmed, doi:10.1016/0092-8674(85)90213-2.
Journal cover image

Published In

Cell

DOI

ISSN

0092-8674

Publication Date

March 1985

Volume

40

Issue

3

Start / End Page

645 / 656

Location

United States

Related Subject Headings

  • Rats, Inbred Strains
  • Rats
  • Protease Inhibitors
  • Phenanthrolines
  • Oligopeptides
  • Metalloendopeptidases
  • Mast Cells
  • Histamine Release
  • Female
  • Exocytosis