Synchronous luminescence: A simple technique for the analysis of hydrolysis activity of the fragile histidine triad protein
Publication
, Journal Article
Askari, M; Miller, G; Vo-Dinh, T
Published in: Biotechnology Letters
January 1, 2001
Human fragile histidine triad (FHIT) protein has dinucleoside 5′,5‴-P1, Pn-polyphosphates hydrolysis activity, with AMP being one of the reaction products. Application of synchronous luminescence (SL) spectroscopy, in which both excitation and emission wavelengths are scanned simultaneously while a constant wavelength interval is maintained between them, was investigated for detection of the enzymatic activity of the FHIT protein. Ability of SL to identify reaction components, AMP production and its increase as a result of increase in substrate concentration and inhibition of the hydrolysis activity by ZnCl2 are demonstrated.
Duke Scholars
Published In
Biotechnology Letters
DOI
ISSN
0141-5492
Publication Date
January 1, 2001
Volume
23
Issue
20
Start / End Page
1697 / 1702
Related Subject Headings
- Biotechnology
- 40 Engineering
- 31 Biological sciences
- 10 Technology
- 09 Engineering
- 06 Biological Sciences
Citation
APA
Chicago
ICMJE
MLA
NLM
Askari, M., Miller, G., & Vo-Dinh, T. (2001). Synchronous luminescence: A simple technique for the analysis of hydrolysis activity of the fragile histidine triad protein. Biotechnology Letters, 23(20), 1697–1702. https://doi.org/10.1023/A:1012404430463
Askari, M., G. Miller, and T. Vo-Dinh. “Synchronous luminescence: A simple technique for the analysis of hydrolysis activity of the fragile histidine triad protein.” Biotechnology Letters 23, no. 20 (January 1, 2001): 1697–1702. https://doi.org/10.1023/A:1012404430463.
Askari M, Miller G, Vo-Dinh T. Synchronous luminescence: A simple technique for the analysis of hydrolysis activity of the fragile histidine triad protein. Biotechnology Letters. 2001 Jan 1;23(20):1697–702.
Askari, M., et al. “Synchronous luminescence: A simple technique for the analysis of hydrolysis activity of the fragile histidine triad protein.” Biotechnology Letters, vol. 23, no. 20, Jan. 2001, pp. 1697–702. Scopus, doi:10.1023/A:1012404430463.
Askari M, Miller G, Vo-Dinh T. Synchronous luminescence: A simple technique for the analysis of hydrolysis activity of the fragile histidine triad protein. Biotechnology Letters. 2001 Jan 1;23(20):1697–1702.
Published In
Biotechnology Letters
DOI
ISSN
0141-5492
Publication Date
January 1, 2001
Volume
23
Issue
20
Start / End Page
1697 / 1702
Related Subject Headings
- Biotechnology
- 40 Engineering
- 31 Biological sciences
- 10 Technology
- 09 Engineering
- 06 Biological Sciences