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Modulation of the transformed and neoplastic phenotype of rat fibroblasts by MHC-I gene expression.

Publication ,  Journal Article
Gattoni-Celli, S; Strauss, RM; Willett, CG; Pozzatti, R; Isselbacher, KJ
Published in: Cancer Res
June 15, 1989

Transfection of the activated ras oncogene (Ha-ras) into second passage rat embryo fibroblasts can induce the metastatic phenotype, while cotransfection of Ha-ras with the adenovirus type 2 E1a gene (Ad2-E1a) yields cells which are tumorigenic but nonmetastatic in nude mice. Because of the presence in nude mice of natural killer cells and B-lymphocytes, which might account for the different metastatic behavior of single versus double transfectants, we used triple deficient mutants as recipient animals in tumorigenicity assays. These mice carry two additional mutations resulting in the deficiency of natural killer cells and activated B-lymphocytes. We observed that the rat embryo fibroblast transfectants exhibit the same metastatic behavior in nude as well as in triple deficient mice, indicating that natural killer and B-cells are not responsible for the observed difference in metastatic phenotype between Ha-ras and Ha-ras plus Ad2-E1a transfectants. Double transfectants were found to express higher levels of major histocompatibility complex class I genes and the degree of expression appeared to correlate inversely with in vitro and in vivo parameters such as the ability to grow in agar-containing semisolid media and rate of tumor formation in triple deficient mice. Our observations are consistent with the concept that expression of major histocompatibility class I genes may be involved in regulating and modifying cell behavior by mechanisms independent of their role in immune recognition.

Duke Scholars

Published In

Cancer Res

ISSN

0008-5472

Publication Date

June 15, 1989

Volume

49

Issue

12

Start / End Page

3392 / 3395

Location

United States

Related Subject Headings

  • Transfection
  • T-Lymphocytes
  • Rats
  • Phenotype
  • Oncology & Carcinogenesis
  • Nucleic Acid Hybridization
  • Neoplasm Metastasis
  • Mice, Nude
  • Mice, Mutant Strains
  • Mice
 

Citation

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Gattoni-Celli, S., Strauss, R. M., Willett, C. G., Pozzatti, R., & Isselbacher, K. J. (1989). Modulation of the transformed and neoplastic phenotype of rat fibroblasts by MHC-I gene expression. Cancer Res, 49(12), 3392–3395.
Gattoni-Celli, S., R. M. Strauss, C. G. Willett, R. Pozzatti, and K. J. Isselbacher. “Modulation of the transformed and neoplastic phenotype of rat fibroblasts by MHC-I gene expression.Cancer Res 49, no. 12 (June 15, 1989): 3392–95.
Gattoni-Celli S, Strauss RM, Willett CG, Pozzatti R, Isselbacher KJ. Modulation of the transformed and neoplastic phenotype of rat fibroblasts by MHC-I gene expression. Cancer Res. 1989 Jun 15;49(12):3392–5.
Gattoni-Celli, S., et al. “Modulation of the transformed and neoplastic phenotype of rat fibroblasts by MHC-I gene expression.Cancer Res, vol. 49, no. 12, June 1989, pp. 3392–95.
Gattoni-Celli S, Strauss RM, Willett CG, Pozzatti R, Isselbacher KJ. Modulation of the transformed and neoplastic phenotype of rat fibroblasts by MHC-I gene expression. Cancer Res. 1989 Jun 15;49(12):3392–3395.

Published In

Cancer Res

ISSN

0008-5472

Publication Date

June 15, 1989

Volume

49

Issue

12

Start / End Page

3392 / 3395

Location

United States

Related Subject Headings

  • Transfection
  • T-Lymphocytes
  • Rats
  • Phenotype
  • Oncology & Carcinogenesis
  • Nucleic Acid Hybridization
  • Neoplasm Metastasis
  • Mice, Nude
  • Mice, Mutant Strains
  • Mice