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Structure and orientation of expressed bovine coronavirus hemagglutinin-esterase protein

Publication ,  Journal Article
Kienzle, TE; Abraham, S; Hogue, BG; Brian, DA
Published in: Journal of Virology
1990

The sequence of the hemagglutinin-esterase (HE) gene for the Mebus strain of bovine coronavirus was obtained from cDNA clones, and its deduced product is a 47,700-kilodalton apoprotein of 424 amino acids. Expression of the HE protein in vitro in the presence of microsomes revealed N-terminal signal peptide cleavage and C-terminal anchorage but not disulfide-linked dimerization. Dimerization was observed only after expression in vivo, during which HE was also transported to the cell surface.

Duke Scholars

Published In

Journal of Virology

ISSN

0022-538X

Publication Date

1990

Volume

64

Issue

4

Start / End Page

1834 / 1838

Related Subject Headings

  • Virology
  • 32 Biomedical and clinical sciences
  • 31 Biological sciences
  • 30 Agricultural, veterinary and food sciences
  • 11 Medical and Health Sciences
  • 07 Agricultural and Veterinary Sciences
  • 06 Biological Sciences
 

Citation

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Kienzle, T. E., Abraham, S., Hogue, B. G., & Brian, D. A. (1990). Structure and orientation of expressed bovine coronavirus hemagglutinin-esterase protein. Journal of Virology, 64(4), 1834–1838.
Kienzle, T. E., S. Abraham, B. G. Hogue, and D. A. Brian. “Structure and orientation of expressed bovine coronavirus hemagglutinin-esterase protein.” Journal of Virology 64, no. 4 (1990): 1834–38.
Kienzle TE, Abraham S, Hogue BG, Brian DA. Structure and orientation of expressed bovine coronavirus hemagglutinin-esterase protein. Journal of Virology. 1990;64(4):1834–8.
Kienzle, T. E., et al. “Structure and orientation of expressed bovine coronavirus hemagglutinin-esterase protein.” Journal of Virology, vol. 64, no. 4, 1990, pp. 1834–38.
Kienzle TE, Abraham S, Hogue BG, Brian DA. Structure and orientation of expressed bovine coronavirus hemagglutinin-esterase protein. Journal of Virology. 1990;64(4):1834–1838.

Published In

Journal of Virology

ISSN

0022-538X

Publication Date

1990

Volume

64

Issue

4

Start / End Page

1834 / 1838

Related Subject Headings

  • Virology
  • 32 Biomedical and clinical sciences
  • 31 Biological sciences
  • 30 Agricultural, veterinary and food sciences
  • 11 Medical and Health Sciences
  • 07 Agricultural and Veterinary Sciences
  • 06 Biological Sciences