Sequencing of double-stranded polymerase chain reaction products for mutation analysis.
Publication
, Journal Article
Anderson, RD; Bao, CY; Minnick, DT; Baxter, J; Veigl, ML; Sedwick, WD
Published in: Mutat Res
July 1993
This report describes a reproducible, straightforward approach to sequencing double-stranded DNA products from the polymerase chain reaction (PCR) for analysis of mutations. The sequencing protocol is a modification of that published by Kretz (Kretz et al., 1989) and has been successful in the hands of a number of investigators working on diverse projects. Following this procedure, PCR DNA products generated from bacterial sources (including pBR322 and F' derivatives), as well as cDNA and genomic DNA from both hamster and human cell lines, have been sequenced with equal success. Close attention to the molar ratio of nucleotides to double-stranded DNA template present during the labeling reaction ensures best results.
Duke Scholars
Published In
Mutat Res
DOI
ISSN
0027-5107
Publication Date
July 1993
Volume
288
Issue
1
Start / End Page
181 / 185
Location
Netherlands
Related Subject Headings
- Templates, Genetic
- Sequence Analysis, DNA
- Polymerase Chain Reaction
- Oncology & Carcinogenesis
- Humans
- Escherichia coli
- Electrophoresis, Agar Gel
- DNA Mutational Analysis
- Cricetinae
- Cell Line
Citation
APA
Chicago
ICMJE
MLA
NLM
Anderson, R. D., Bao, C. Y., Minnick, D. T., Baxter, J., Veigl, M. L., & Sedwick, W. D. (1993). Sequencing of double-stranded polymerase chain reaction products for mutation analysis. Mutat Res, 288(1), 181–185. https://doi.org/10.1016/0027-5107(93)90219-6
Anderson, R. D., C. Y. Bao, D. T. Minnick, J. Baxter, M. L. Veigl, and W. D. Sedwick. “Sequencing of double-stranded polymerase chain reaction products for mutation analysis.” Mutat Res 288, no. 1 (July 1993): 181–85. https://doi.org/10.1016/0027-5107(93)90219-6.
Anderson RD, Bao CY, Minnick DT, Baxter J, Veigl ML, Sedwick WD. Sequencing of double-stranded polymerase chain reaction products for mutation analysis. Mutat Res. 1993 Jul;288(1):181–5.
Anderson, R. D., et al. “Sequencing of double-stranded polymerase chain reaction products for mutation analysis.” Mutat Res, vol. 288, no. 1, July 1993, pp. 181–85. Pubmed, doi:10.1016/0027-5107(93)90219-6.
Anderson RD, Bao CY, Minnick DT, Baxter J, Veigl ML, Sedwick WD. Sequencing of double-stranded polymerase chain reaction products for mutation analysis. Mutat Res. 1993 Jul;288(1):181–185.
Published In
Mutat Res
DOI
ISSN
0027-5107
Publication Date
July 1993
Volume
288
Issue
1
Start / End Page
181 / 185
Location
Netherlands
Related Subject Headings
- Templates, Genetic
- Sequence Analysis, DNA
- Polymerase Chain Reaction
- Oncology & Carcinogenesis
- Humans
- Escherichia coli
- Electrophoresis, Agar Gel
- DNA Mutational Analysis
- Cricetinae
- Cell Line