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Failure of red blood cell maturation in mice with defects in the high-density lipoprotein receptor SR-BI.

Publication ,  Journal Article
Holm, TM; Braun, A; Trigatti, BL; Brugnara, C; Sakamoto, M; Krieger, M; Andrews, NC
Published in: Blood
March 2002

Mammalian erythrocytes undergo a unique maturation process in which they discard their nuclei and organelles and assume a flexible biconcave shape. We found that altered plasma lipoprotein metabolism can profoundly influence these events. Abnormal erythrocyte morphology was observed in hypercholesterolemic mice lacking the high-density lipoprotein receptor SR-BI. This was exacerbated by feeding mice a high-cholesterol diet or, more dramatically, by inactivating the apolipoprotein E gene. Erythrocytes from SR-BI(-/-)/apolipoprotein E(-/-) mice and SR-BI(-/-) mice that were fed cholesterol had markedly increased membrane cholesterol. Their morphology appeared immature, with macrocytosis, irregular shape, and large autophagolysosomes. Autophagolysosomes from SR-BI(-/-)/apolipoprotein E(-/-) erythrocytes were expelled when the erythrocytes were transfused into wild-type animals or incubated in vitro with normolipidemic serum or the cholesterol-sequestering agent methyl cyclodextrin. We propose that autophagocytosis and phagolysosome expulsion are essential steps in erythroid maturation and that expulsion is inhibited in the presence of markedly increased cellular cholesterol.

Published In

Blood

DOI

EISSN

1528-0020

ISSN

0006-4971

Publication Date

March 2002

Volume

99

Issue

5

Start / End Page

1817 / 1824

Related Subject Headings

  • Scavenger Receptors, Class B
  • Reticulocytes
  • Receptors, Scavenger
  • Receptors, Lipoprotein
  • Receptors, Immunologic
  • RNA-Binding Proteins
  • Phagosomes
  • Mice, Knockout
  • Mice
  • Membrane Proteins
 

Citation

APA
Chicago
ICMJE
MLA
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Holm, T. M., Braun, A., Trigatti, B. L., Brugnara, C., Sakamoto, M., Krieger, M., & Andrews, N. C. (2002). Failure of red blood cell maturation in mice with defects in the high-density lipoprotein receptor SR-BI. Blood, 99(5), 1817–1824. https://doi.org/10.1182/blood.v99.5.1817
Holm, Teresa M., Anne Braun, Bernardo L. Trigatti, Carlo Brugnara, Masa Sakamoto, Monty Krieger, and Nancy C. Andrews. “Failure of red blood cell maturation in mice with defects in the high-density lipoprotein receptor SR-BI.Blood 99, no. 5 (March 2002): 1817–24. https://doi.org/10.1182/blood.v99.5.1817.
Holm TM, Braun A, Trigatti BL, Brugnara C, Sakamoto M, Krieger M, et al. Failure of red blood cell maturation in mice with defects in the high-density lipoprotein receptor SR-BI. Blood. 2002 Mar;99(5):1817–24.
Holm, Teresa M., et al. “Failure of red blood cell maturation in mice with defects in the high-density lipoprotein receptor SR-BI.Blood, vol. 99, no. 5, Mar. 2002, pp. 1817–24. Epmc, doi:10.1182/blood.v99.5.1817.
Holm TM, Braun A, Trigatti BL, Brugnara C, Sakamoto M, Krieger M, Andrews NC. Failure of red blood cell maturation in mice with defects in the high-density lipoprotein receptor SR-BI. Blood. 2002 Mar;99(5):1817–1824.

Published In

Blood

DOI

EISSN

1528-0020

ISSN

0006-4971

Publication Date

March 2002

Volume

99

Issue

5

Start / End Page

1817 / 1824

Related Subject Headings

  • Scavenger Receptors, Class B
  • Reticulocytes
  • Receptors, Scavenger
  • Receptors, Lipoprotein
  • Receptors, Immunologic
  • RNA-Binding Proteins
  • Phagosomes
  • Mice, Knockout
  • Mice
  • Membrane Proteins