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Noninvasive visualization of the choriocapillaris and its dynamic filling.

Publication ,  Journal Article
Kiryu, J; Shahidi, M; Mori, MT; Ogura, Y; Asrani, S; Zeimer, R
Published in: Invest Ophthalmol Vis Sci
September 1994

PURPOSE: The choroidal microvasculature and its circulation are inadequately assessed by presently available techniques. Laser-targeted delivery was applied to generate local, repetitive angiograms of the choriocapillaris in primates. METHODS: Carboxyfluorescein was encapsulated in heat-sensitive liposomes and injected intravenously in monkeys. The liposome contents were then released locally in the choroid by application of a short heat pulse provided by an infrared laser. The bolus of dye spread rapidly downstream from the underlying arterioles into clusters of lobules. Video angiograms were generated with excitation illumination provided by an argon laser. RESULTS: Laser-targeted delivery choroidal angiography performed on three monkeys indicated that the fluorescence was emitted mainly from the choriocapillaris. Clusters of irregular shape with well-defined margins were observed. Adjacent arteries typically supplied separate clusters that fit together like a jigsaw puzzle. The dynamic filling and emptying patterns, recorded at video rate, revealed that macular lobules were filled by a central arteriole and drained by a venous annulus. The average dye transit time through a lobule (n = 10) was 118 +/- 26 msec (mean +/- SD), and the dye transit velocity was 2.53 +/- 0.55 mm/sec. CONCLUSIONS: This study clearly documents the segmental nature of the primate choroidal microvasculature. It also illustrates that choroidal angiography by laser-targeted dye delivery provides information useful for studying the response of the choriocapillaris to physiological and pathologic changes.

Duke Scholars

Published In

Invest Ophthalmol Vis Sci

ISSN

0146-0404

Publication Date

September 1994

Volume

35

Issue

10

Start / End Page

3724 / 3731

Location

United States

Related Subject Headings

  • Regional Blood Flow
  • Papio
  • Ophthalmology & Optometry
  • Liposomes
  • Lasers
  • Fundus Oculi
  • Fluoresceins
  • Fluorescein Angiography
  • Drug Carriers
  • Choroid
 

Citation

APA
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ICMJE
MLA
NLM
Kiryu, J., Shahidi, M., Mori, M. T., Ogura, Y., Asrani, S., & Zeimer, R. (1994). Noninvasive visualization of the choriocapillaris and its dynamic filling. Invest Ophthalmol Vis Sci, 35(10), 3724–3731.
Kiryu, J., M. Shahidi, M. T. Mori, Y. Ogura, S. Asrani, and R. Zeimer. “Noninvasive visualization of the choriocapillaris and its dynamic filling.Invest Ophthalmol Vis Sci 35, no. 10 (September 1994): 3724–31.
Kiryu J, Shahidi M, Mori MT, Ogura Y, Asrani S, Zeimer R. Noninvasive visualization of the choriocapillaris and its dynamic filling. Invest Ophthalmol Vis Sci. 1994 Sep;35(10):3724–31.
Kiryu, J., et al. “Noninvasive visualization of the choriocapillaris and its dynamic filling.Invest Ophthalmol Vis Sci, vol. 35, no. 10, Sept. 1994, pp. 3724–31.
Kiryu J, Shahidi M, Mori MT, Ogura Y, Asrani S, Zeimer R. Noninvasive visualization of the choriocapillaris and its dynamic filling. Invest Ophthalmol Vis Sci. 1994 Sep;35(10):3724–3731.

Published In

Invest Ophthalmol Vis Sci

ISSN

0146-0404

Publication Date

September 1994

Volume

35

Issue

10

Start / End Page

3724 / 3731

Location

United States

Related Subject Headings

  • Regional Blood Flow
  • Papio
  • Ophthalmology & Optometry
  • Liposomes
  • Lasers
  • Fundus Oculi
  • Fluoresceins
  • Fluorescein Angiography
  • Drug Carriers
  • Choroid