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Nuclear Snail1 and nuclear ZEB1 protein expression in invasive and intraductal human breast carcinomas.

Publication ,  Journal Article
Geradts, J; de Herreros, AG; Su, Z; Burchette, J; Broadwater, G; Bachelder, RE
Published in: Hum Pathol
August 2011

Snail1 and ZEB1 are transcriptional repressors that drive tumor initiation and metastasis in animal models. Snail1 and ZEB1 are frequently coexpressed in tumor cell lines, suggesting that these factors may cooperate to promote tumor progression. However, coexpression of these transcriptional repressors in primary human cancer specimens has not been investigated. Previous studies assessed expression in primary breast cancers of Snail1 messenger RNA, which does not reflect Snail1 activity because Snail1 is subject to posttranslational modifications that inhibit its nuclear localization/activity. In the current study, using breast tumor cell lines of known Snail1 and ZEB1 expression status, we developed immunohistochemistry protocols for detecting nuclear Snail1 and nuclear ZEB1 proteins. Using these protocols, we assessed nuclear Snail1 and nuclear ZEB1 expressions in primary human breast cancers of varying subtypes (n = 78). Nuclear Snail1 and estrogen receptor α expressions were inversely associated in primary breast cancers, and nuclear Snail1 was expressed in approximately 80% of triple-negative breast cancers (lacking estrogen receptor α, progesterone receptor, and human epidermal growth factor receptor 2 overexpression). In contrast, nuclear ZEB1 was expressed at a significantly lower frequency in these breast cancers. Notably, nuclear Snail1 protein was detected in 45% of ductal carcinoma in situ specimens (n = 29), raising the important possibility that nuclear Snail1 expression in early stage breast lesions may predict future development of invasive breast cancer. Collectively, our studies demonstrate frequent expression of nuclear Snail1, but not nuclear ZEB1, in invasive, triple-negative breast cancers as well as in intraductal carcinomas.

Duke Scholars

Published In

Hum Pathol

DOI

EISSN

1532-8392

Publication Date

August 2011

Volume

42

Issue

8

Start / End Page

1125 / 1131

Location

United States

Related Subject Headings

  • Zinc Finger E-box-Binding Homeobox 1
  • Transcription Factors
  • Tissue Array Analysis
  • Snail Family Transcription Factors
  • Prognosis
  • Pathology
  • Humans
  • Homeodomain Proteins
  • Female
  • Cell Nucleus
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Geradts, J., de Herreros, A. G., Su, Z., Burchette, J., Broadwater, G., & Bachelder, R. E. (2011). Nuclear Snail1 and nuclear ZEB1 protein expression in invasive and intraductal human breast carcinomas. Hum Pathol, 42(8), 1125–1131. https://doi.org/10.1016/j.humpath.2010.11.004
Geradts, Joseph, Antonio Garcia de Herreros, Zuowei Su, James Burchette, Gloria Broadwater, and Robin E. Bachelder. “Nuclear Snail1 and nuclear ZEB1 protein expression in invasive and intraductal human breast carcinomas.Hum Pathol 42, no. 8 (August 2011): 1125–31. https://doi.org/10.1016/j.humpath.2010.11.004.
Geradts J, de Herreros AG, Su Z, Burchette J, Broadwater G, Bachelder RE. Nuclear Snail1 and nuclear ZEB1 protein expression in invasive and intraductal human breast carcinomas. Hum Pathol. 2011 Aug;42(8):1125–31.
Geradts, Joseph, et al. “Nuclear Snail1 and nuclear ZEB1 protein expression in invasive and intraductal human breast carcinomas.Hum Pathol, vol. 42, no. 8, Aug. 2011, pp. 1125–31. Pubmed, doi:10.1016/j.humpath.2010.11.004.
Geradts J, de Herreros AG, Su Z, Burchette J, Broadwater G, Bachelder RE. Nuclear Snail1 and nuclear ZEB1 protein expression in invasive and intraductal human breast carcinomas. Hum Pathol. 2011 Aug;42(8):1125–1131.
Journal cover image

Published In

Hum Pathol

DOI

EISSN

1532-8392

Publication Date

August 2011

Volume

42

Issue

8

Start / End Page

1125 / 1131

Location

United States

Related Subject Headings

  • Zinc Finger E-box-Binding Homeobox 1
  • Transcription Factors
  • Tissue Array Analysis
  • Snail Family Transcription Factors
  • Prognosis
  • Pathology
  • Humans
  • Homeodomain Proteins
  • Female
  • Cell Nucleus