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Genetic control of single lumen formation in the zebrafish gut.

Publication ,  Journal Article
Bagnat, M; Cheung, ID; Mostov, KE; Stainier, DYR
Published in: Nat Cell Biol
August 2007

Most organs consist of networks of interconnected tubes that serve as conduits to transport fluid and cells and act as physiological barriers between compartments. Biological tubes are assembled through very diverse developmental processes that generate structures of different shapes and sizes. Nevertheless, all biological tubes invariably possess one single lumen. The mechanisms responsible for single lumen specification are not known. Here we show that zebrafish mutants for the MODY5 and familial GCKD gene tcf2 (also known as vhnf1) fail to specify a single lumen in their gut tube and instead develop multiple lumens. We show that Tcf2 controls single lumen formation by regulating claudin15 and Na+/K+-ATPase expression. Our in vivo and in vitro results indicate that Claudin15 functions in paracellular ion transport to specify single lumen formation. This work shows that single lumen formation is genetically controlled and appears to be driven by the accumulation of fluid.

Duke Scholars

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Published In

Nat Cell Biol

DOI

ISSN

1465-7392

Publication Date

August 2007

Volume

9

Issue

8

Start / End Page

954 / 960

Location

England

Related Subject Headings

  • Zebrafish Proteins
  • Zebrafish
  • Sodium-Potassium-Exchanging ATPase
  • Morphogenesis
  • Molecular Sequence Data
  • Membrane Proteins
  • Ion Transport
  • Ion Channels
  • Intestines
  • In Situ Hybridization
 

Citation

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Bagnat, M., Cheung, I. D., Mostov, K. E., & Stainier, D. Y. R. (2007). Genetic control of single lumen formation in the zebrafish gut. Nat Cell Biol, 9(8), 954–960. https://doi.org/10.1038/ncb1621
Bagnat, Michel, Isla D. Cheung, Keith E. Mostov, and Didier Y. R. Stainier. “Genetic control of single lumen formation in the zebrafish gut.Nat Cell Biol 9, no. 8 (August 2007): 954–60. https://doi.org/10.1038/ncb1621.
Bagnat M, Cheung ID, Mostov KE, Stainier DYR. Genetic control of single lumen formation in the zebrafish gut. Nat Cell Biol. 2007 Aug;9(8):954–60.
Bagnat, Michel, et al. “Genetic control of single lumen formation in the zebrafish gut.Nat Cell Biol, vol. 9, no. 8, Aug. 2007, pp. 954–60. Pubmed, doi:10.1038/ncb1621.
Bagnat M, Cheung ID, Mostov KE, Stainier DYR. Genetic control of single lumen formation in the zebrafish gut. Nat Cell Biol. 2007 Aug;9(8):954–960.

Published In

Nat Cell Biol

DOI

ISSN

1465-7392

Publication Date

August 2007

Volume

9

Issue

8

Start / End Page

954 / 960

Location

England

Related Subject Headings

  • Zebrafish Proteins
  • Zebrafish
  • Sodium-Potassium-Exchanging ATPase
  • Morphogenesis
  • Molecular Sequence Data
  • Membrane Proteins
  • Ion Transport
  • Ion Channels
  • Intestines
  • In Situ Hybridization