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OP18/stathmin binds near the C-terminus of tubulin and facilitates GTP binding.

Publication ,  Journal Article
Moreno, FJ; Bagnat, M; Lim, F; Avila, J
Published in: Eur J Biochem
June 1999

It is has been previously suggested that the protein Op18/stathmin may interact with tubulin via the alpha-tubulin subunit [Larsson, N., Marklund, U., Melander Gradin, H., Brattsand, G. & Gullberg, M. (1997) Mol. Cell. Biol. 17, 5530-5539]. In this study we have used limited proteolysis and cross-linking analysis to localize further the stathmin-binding site on alpha-tubulin. Our results indicate that such a binding site is in a region close to the C-terminus of the molecule comprising residues 307 to the subtilisin-cleavage site on the alpha-tubulin subunit. Based on a recent model of the structure of tubulin [Nogales, E., Wolf, S.G. & Dowing, D.H. (1998) Nature (London) 391, 199-203], we found that this region contained the same areas that may be involved in longitudinal contacts of alpha-tubulin subunits within the microtubule. We also observed that the binding of stathmin to tubulin can modulate the binding of GTP to tubulin, as a consequence of a conformational change in the beta-tubulin subunit that occurs upon interaction of stathmin with tubulin.

Duke Scholars

Published In

Eur J Biochem

DOI

ISSN

0014-2956

Publication Date

June 1999

Volume

262

Issue

2

Start / End Page

557 / 562

Location

England

Related Subject Headings

  • Tubulin
  • Stathmin
  • Protein Binding
  • Phosphoproteins
  • Microtubule Proteins
  • Cattle
  • Biochemistry & Molecular Biology
  • Binding Sites
  • Animals
  • Adenosine Triphosphate
 

Citation

APA
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ICMJE
MLA
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Moreno, F. J., Bagnat, M., Lim, F., & Avila, J. (1999). OP18/stathmin binds near the C-terminus of tubulin and facilitates GTP binding. Eur J Biochem, 262(2), 557–562. https://doi.org/10.1046/j.1432-1327.1999.00408.x
Moreno, F. J., M. Bagnat, F. Lim, and J. Avila. “OP18/stathmin binds near the C-terminus of tubulin and facilitates GTP binding.Eur J Biochem 262, no. 2 (June 1999): 557–62. https://doi.org/10.1046/j.1432-1327.1999.00408.x.
Moreno FJ, Bagnat M, Lim F, Avila J. OP18/stathmin binds near the C-terminus of tubulin and facilitates GTP binding. Eur J Biochem. 1999 Jun;262(2):557–62.
Moreno, F. J., et al. “OP18/stathmin binds near the C-terminus of tubulin and facilitates GTP binding.Eur J Biochem, vol. 262, no. 2, June 1999, pp. 557–62. Pubmed, doi:10.1046/j.1432-1327.1999.00408.x.
Moreno FJ, Bagnat M, Lim F, Avila J. OP18/stathmin binds near the C-terminus of tubulin and facilitates GTP binding. Eur J Biochem. 1999 Jun;262(2):557–562.

Published In

Eur J Biochem

DOI

ISSN

0014-2956

Publication Date

June 1999

Volume

262

Issue

2

Start / End Page

557 / 562

Location

England

Related Subject Headings

  • Tubulin
  • Stathmin
  • Protein Binding
  • Phosphoproteins
  • Microtubule Proteins
  • Cattle
  • Biochemistry & Molecular Biology
  • Binding Sites
  • Animals
  • Adenosine Triphosphate