Glycophase glass revisited: protein adsorption and cell growth on glass surfaces bearing immobilized glycerol monosaccharides.
Publication
, Journal Article
Bain, JR; Hoffman, AS
Published in: Biomaterials
August 2002
Gamma-Glycerylpropylsilyl or "glycophase" glass has been promoted as a non-fouling surface, resistant to protein adsorption and cell attachment, on which one can immobilize oligopeptide ligands, and thus create cell-type-specific culture surfaces. The present study confirmed that the glycerol-rich glycophase surface is a useful support for peptide immobilization. But glycophase glass was observed to adsorb more albumin than glass. At pH 7.4, desorption studies revealed that albumin bound more tightly to glycophase glass than to glass. Moreover, the growth rates, morphologies, and functions of transgenic betaG I/17 insulinoma cell cultures were equivalent on the two surfaces. Glycophase glass is neither protein- nor cell-repellant.
Duke Scholars
Published In
Biomaterials
DOI
ISSN
0142-9612
Publication Date
August 2002
Volume
23
Issue
16
Start / End Page
3347 / 3357
Location
Netherlands
Related Subject Headings
- Tumor Cells, Cultured
- Silicates
- Serum Albumin, Bovine
- Pancreatic Neoplasms
- Oligopeptides
- Monosaccharides
- Molecular Structure
- Insulinoma
- Insulin Secretion
- Insulin
Citation
APA
Chicago
ICMJE
MLA
NLM
Bain, J. R., & Hoffman, A. S. (2002). Glycophase glass revisited: protein adsorption and cell growth on glass surfaces bearing immobilized glycerol monosaccharides. Biomaterials, 23(16), 3347–3357. https://doi.org/10.1016/s0142-9612(02)00035-2
Bain, James R., and Allan S. Hoffman. “Glycophase glass revisited: protein adsorption and cell growth on glass surfaces bearing immobilized glycerol monosaccharides.” Biomaterials 23, no. 16 (August 2002): 3347–57. https://doi.org/10.1016/s0142-9612(02)00035-2.
Bain JR, Hoffman AS. Glycophase glass revisited: protein adsorption and cell growth on glass surfaces bearing immobilized glycerol monosaccharides. Biomaterials. 2002 Aug;23(16):3347–57.
Bain, James R., and Allan S. Hoffman. “Glycophase glass revisited: protein adsorption and cell growth on glass surfaces bearing immobilized glycerol monosaccharides.” Biomaterials, vol. 23, no. 16, Aug. 2002, pp. 3347–57. Pubmed, doi:10.1016/s0142-9612(02)00035-2.
Bain JR, Hoffman AS. Glycophase glass revisited: protein adsorption and cell growth on glass surfaces bearing immobilized glycerol monosaccharides. Biomaterials. 2002 Aug;23(16):3347–3357.
Published In
Biomaterials
DOI
ISSN
0142-9612
Publication Date
August 2002
Volume
23
Issue
16
Start / End Page
3347 / 3357
Location
Netherlands
Related Subject Headings
- Tumor Cells, Cultured
- Silicates
- Serum Albumin, Bovine
- Pancreatic Neoplasms
- Oligopeptides
- Monosaccharides
- Molecular Structure
- Insulinoma
- Insulin Secretion
- Insulin