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Detection of minor drug-resistant populations by parallel allele-specific sequencing.

Publication ,  Journal Article
Cai, F; Chen, H; Hicks, CB; Bartlett, JA; Zhu, J; Gao, F
Published in: Nat Methods
February 2007

We developed a highly sensitive parallel allele-specific sequencing (PASS) assay to simultaneously analyze a large number of viral genomes and detect minor drug-resistant populations at approximately 0.1-0.01% levels. Using this assay on samples from individuals infected with human immunodeficiency viruses (HIV), we successfully detected and quantified minor populations of drug-resistant viruses and performed linkage analysis of multiple-drug resistance mutations. This assay may serve as a useful tool to study drug resistance in HIV and other infectious agents.

Duke Scholars

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Published In

Nat Methods

DOI

ISSN

1548-7091

Publication Date

February 2007

Volume

4

Issue

2

Start / End Page

123 / 125

Location

United States

Related Subject Headings

  • Treatment Failure
  • Sensitivity and Specificity
  • Polymerase Chain Reaction
  • Mutation
  • Humans
  • HIV Infections
  • HIV
  • Genome, Viral
  • Genetic Linkage
  • Drug Resistance, Viral
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Cai, F., Chen, H., Hicks, C. B., Bartlett, J. A., Zhu, J., & Gao, F. (2007). Detection of minor drug-resistant populations by parallel allele-specific sequencing. Nat Methods, 4(2), 123–125. https://doi.org/10.1038/nmeth995
Cai, Fangping, Haifeng Chen, Charles B. Hicks, John A. Bartlett, Jun Zhu, and Feng Gao. “Detection of minor drug-resistant populations by parallel allele-specific sequencing.Nat Methods 4, no. 2 (February 2007): 123–25. https://doi.org/10.1038/nmeth995.
Cai F, Chen H, Hicks CB, Bartlett JA, Zhu J, Gao F. Detection of minor drug-resistant populations by parallel allele-specific sequencing. Nat Methods. 2007 Feb;4(2):123–5.
Cai, Fangping, et al. “Detection of minor drug-resistant populations by parallel allele-specific sequencing.Nat Methods, vol. 4, no. 2, Feb. 2007, pp. 123–25. Pubmed, doi:10.1038/nmeth995.
Cai F, Chen H, Hicks CB, Bartlett JA, Zhu J, Gao F. Detection of minor drug-resistant populations by parallel allele-specific sequencing. Nat Methods. 2007 Feb;4(2):123–125.

Published In

Nat Methods

DOI

ISSN

1548-7091

Publication Date

February 2007

Volume

4

Issue

2

Start / End Page

123 / 125

Location

United States

Related Subject Headings

  • Treatment Failure
  • Sensitivity and Specificity
  • Polymerase Chain Reaction
  • Mutation
  • Humans
  • HIV Infections
  • HIV
  • Genome, Viral
  • Genetic Linkage
  • Drug Resistance, Viral