Induction of oxidative cell damage by photo-treatment with zinc meta N-methylpyridylporphyrin.
We have previously reported that isomeric Zn(II) N-methylpyridylporphyrins (ZnTM-2(3,4)-PyP4 + ) can act as photosensitizers with efficacy comparable to that of hematoporphyrin derivative (HpD) in preventing cell proliferation and causing cell death in vitro. To better understand the biochemical basis of this activity, the effects of photo-activated ZnTM-3-PyP4 + on GSH/GSSG ratio, lipid peroxidation, membrane permeability, oxidative DNA damage, and the activities of SOD, catalase, glutathione reductase, and glutathione peroxidase were evaluated. Light exposure of ZnTM-3-PyP4 + -treated colon adenocarcinoma cells caused a wide spectrum of oxidative damage including depletion of GSH, inactivation of glutathione reductase and glutathione peroxidase, oxidative DNA damage and peroxidation of membrane lipids. Cell staining with Hoechst-33342 showed morphological changes consistent with both necrotic and apoptotic death sequences, depending upon the presence of oxygen.
Duke Scholars
Published In
DOI
ISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Superoxide Dismutase
- Photosensitizing Agents
- Photosensitivity Disorders
- Oxidative Stress
- Metalloporphyrins
- Lipid Peroxidation
- Humans
- Glutathione Reductase
- Glutathione Peroxidase
- Glutathione Disulfide
Citation
Published In
DOI
ISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Superoxide Dismutase
- Photosensitizing Agents
- Photosensitivity Disorders
- Oxidative Stress
- Metalloporphyrins
- Lipid Peroxidation
- Humans
- Glutathione Reductase
- Glutathione Peroxidase
- Glutathione Disulfide