Skip to main content
Journal cover image

Regulation of skeletal muscle oxidative capacity and insulin signaling by the mitochondrial rhomboid protease PARL.

Publication ,  Journal Article
Civitarese, AE; MacLean, PS; Carling, S; Kerr-Bayles, L; McMillan, RP; Pierce, A; Becker, TC; Moro, C; Finlayson, J; Lefort, N; Newgard, CB ...
Published in: Cell Metab
May 5, 2010

Type 2 diabetes mellitus (T2DM) and aging are characterized by insulin resistance and impaired mitochondrial energetics. In lower organisms, remodeling by the protease pcp1 (PARL ortholog) maintains the function and lifecycle of mitochondria. We examined whether variation in PARL protein content is associated with mitochondrial abnormalities and insulin resistance. PARL mRNA and mitochondrial mass were both reduced in elderly subjects and in subjects with T2DM. Muscle knockdown of PARL in mice resulted in malformed mitochondrial cristae, lower mitochondrial content, decreased PGC1alpha protein levels, and impaired insulin signaling. Suppression of PARL protein in healthy myotubes lowered mitochondrial mass and insulin-stimulated glycogen synthesis and increased reactive oxygen species production. We propose that lower PARL expression may contribute to the mitochondrial abnormalities seen in aging and T2DM.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Cell Metab

DOI

EISSN

1932-7420

Publication Date

May 5, 2010

Volume

11

Issue

5

Start / End Page

412 / 426

Location

United States

Related Subject Headings

  • Transcription Factors
  • Trans-Activators
  • Signal Transduction
  • Reactive Oxygen Species
  • Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha
  • Muscle, Skeletal
  • Mitochondrial Proteins
  • Mitochondria
  • Middle Aged
  • Mice, Knockout
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Civitarese, A. E., MacLean, P. S., Carling, S., Kerr-Bayles, L., McMillan, R. P., Pierce, A., … Ravussin, E. (2010). Regulation of skeletal muscle oxidative capacity and insulin signaling by the mitochondrial rhomboid protease PARL. Cell Metab, 11(5), 412–426. https://doi.org/10.1016/j.cmet.2010.04.004
Civitarese, Anthony E., Paul S. MacLean, Stacy Carling, Lyndal Kerr-Bayles, Ryan P. McMillan, Anson Pierce, Thomas C. Becker, et al. “Regulation of skeletal muscle oxidative capacity and insulin signaling by the mitochondrial rhomboid protease PARL.Cell Metab 11, no. 5 (May 5, 2010): 412–26. https://doi.org/10.1016/j.cmet.2010.04.004.
Civitarese AE, MacLean PS, Carling S, Kerr-Bayles L, McMillan RP, Pierce A, et al. Regulation of skeletal muscle oxidative capacity and insulin signaling by the mitochondrial rhomboid protease PARL. Cell Metab. 2010 May 5;11(5):412–26.
Civitarese, Anthony E., et al. “Regulation of skeletal muscle oxidative capacity and insulin signaling by the mitochondrial rhomboid protease PARL.Cell Metab, vol. 11, no. 5, May 2010, pp. 412–26. Pubmed, doi:10.1016/j.cmet.2010.04.004.
Civitarese AE, MacLean PS, Carling S, Kerr-Bayles L, McMillan RP, Pierce A, Becker TC, Moro C, Finlayson J, Lefort N, Newgard CB, Mandarino L, Cefalu W, Walder K, Collier GR, Hulver MW, Smith SR, Ravussin E. Regulation of skeletal muscle oxidative capacity and insulin signaling by the mitochondrial rhomboid protease PARL. Cell Metab. 2010 May 5;11(5):412–426.
Journal cover image

Published In

Cell Metab

DOI

EISSN

1932-7420

Publication Date

May 5, 2010

Volume

11

Issue

5

Start / End Page

412 / 426

Location

United States

Related Subject Headings

  • Transcription Factors
  • Trans-Activators
  • Signal Transduction
  • Reactive Oxygen Species
  • Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha
  • Muscle, Skeletal
  • Mitochondrial Proteins
  • Mitochondria
  • Middle Aged
  • Mice, Knockout