The Tetrahymena ribozyme acts like an RNA restriction endonuclease.
Publication
, Journal Article
Zaug, AJ; Been, MD; Cech, TR
Published in: Nature
December 4, 1986
A shortened form of the Tetrahymena self-splicing ribosomal RNA intervening sequence acts as an endoribonuclease, catalysing the cleavage of large RNA molecules by a mechanism involving guanosine transfer. The sequence specificity approaches that of the DNA restriction endonucleases. Site-specific mutagenesis of the enzyme active site alters the substrate sequence specificity in a predictable manner, so that endoribonucleases can be synthesized to cut at a variety of tetranucleotide sequences.
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Published In
Nature
DOI
ISSN
0028-0836
Publication Date
December 4, 1986
Volume
324
Issue
6096
Start / End Page
429 / 433
Location
England
Related Subject Headings
- Tetrahymena
- Substrate Specificity
- RNA, Ribosomal
- RNA, Catalytic
- RNA Splicing
- Mutation
- Kinetics
- General Science & Technology
- Binding Sites
- Base Sequence
Citation
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Chicago
ICMJE
MLA
NLM
Zaug, A. J., Been, M. D., & Cech, T. R. (1986). The Tetrahymena ribozyme acts like an RNA restriction endonuclease. Nature, 324(6096), 429–433. https://doi.org/10.1038/324429a0
Zaug, A. J., M. D. Been, and T. R. Cech. “The Tetrahymena ribozyme acts like an RNA restriction endonuclease.” Nature 324, no. 6096 (December 4, 1986): 429–33. https://doi.org/10.1038/324429a0.
Zaug AJ, Been MD, Cech TR. The Tetrahymena ribozyme acts like an RNA restriction endonuclease. Nature. 1986 Dec 4;324(6096):429–33.
Zaug, A. J., et al. “The Tetrahymena ribozyme acts like an RNA restriction endonuclease.” Nature, vol. 324, no. 6096, Dec. 1986, pp. 429–33. Pubmed, doi:10.1038/324429a0.
Zaug AJ, Been MD, Cech TR. The Tetrahymena ribozyme acts like an RNA restriction endonuclease. Nature. 1986 Dec 4;324(6096):429–433.
Published In
Nature
DOI
ISSN
0028-0836
Publication Date
December 4, 1986
Volume
324
Issue
6096
Start / End Page
429 / 433
Location
England
Related Subject Headings
- Tetrahymena
- Substrate Specificity
- RNA, Ribosomal
- RNA, Catalytic
- RNA Splicing
- Mutation
- Kinetics
- General Science & Technology
- Binding Sites
- Base Sequence