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Negative regulation of Armadillo, a Wingless effector in Drosophila.

Publication ,  Journal Article
Pai, LM; Orsulic, S; Bejsovec, A; Peifer, M
Published in: Development (Cambridge, England)
June 1997

Drosophila Armadillo and its vertebrate homolog beta-catenin play essential roles both in the transduction of Wingless/Wnt cell-cell signals and in the function of cell-cell adherens junctions. Wingless and Wnts direct numerous cell fate choices during development. We generated a mutant protein, Armadillo(S10), with a 54 amino acid deletion in its N-terminal domain. This mutant is constitutively active in Wingless signaling; its activity is independent of both Wingless signal and endogenous wild-type Armadillo. Armadillo's role in signal transduction is normally negatively regulated by Zeste-white 3 kinase, which modulates Armadillo protein stability. Armadillo(S10) is more stable than wild-type Armadillo, suggesting that it is less rapidly targeted for degradation. We show that Armadillo(S10) has escaped from negative regulation by Zeste white-3 kinase, and thus accumulates outside junctions even in the absence of Wingless signal. Finally, we present data implicating kinases in addition to Zeste white-3 in Armadillo phosphorylation. We discuss two models for the negative regulation of Armadillo in normal development and discuss how escape from this regulation contributes to tumorigenesis.

Duke Scholars

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Published In

Development (Cambridge, England)

DOI

EISSN

1477-9129

ISSN

0950-1991

Publication Date

June 1997

Volume

124

Issue

11

Start / End Page

2255 / 2266

Related Subject Headings

  • Wnt1 Protein
  • Transcription Factors
  • Trans-Activators
  • Signal Transduction
  • Sequence Deletion
  • Recombinant Fusion Proteins
  • Proto-Oncogene Proteins
  • Protein Serine-Threonine Kinases
  • Phosphorylation
  • Molecular Sequence Data
 

Citation

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Pai, L. M., Orsulic, S., Bejsovec, A., & Peifer, M. (1997). Negative regulation of Armadillo, a Wingless effector in Drosophila. Development (Cambridge, England), 124(11), 2255–2266. https://doi.org/10.1242/dev.124.11.2255
Pai, L. M., S. Orsulic, A. Bejsovec, and M. Peifer. “Negative regulation of Armadillo, a Wingless effector in Drosophila.Development (Cambridge, England) 124, no. 11 (June 1997): 2255–66. https://doi.org/10.1242/dev.124.11.2255.
Pai LM, Orsulic S, Bejsovec A, Peifer M. Negative regulation of Armadillo, a Wingless effector in Drosophila. Development (Cambridge, England). 1997 Jun;124(11):2255–66.
Pai, L. M., et al. “Negative regulation of Armadillo, a Wingless effector in Drosophila.Development (Cambridge, England), vol. 124, no. 11, June 1997, pp. 2255–66. Epmc, doi:10.1242/dev.124.11.2255.
Pai LM, Orsulic S, Bejsovec A, Peifer M. Negative regulation of Armadillo, a Wingless effector in Drosophila. Development (Cambridge, England). 1997 Jun;124(11):2255–2266.
Journal cover image

Published In

Development (Cambridge, England)

DOI

EISSN

1477-9129

ISSN

0950-1991

Publication Date

June 1997

Volume

124

Issue

11

Start / End Page

2255 / 2266

Related Subject Headings

  • Wnt1 Protein
  • Transcription Factors
  • Trans-Activators
  • Signal Transduction
  • Sequence Deletion
  • Recombinant Fusion Proteins
  • Proto-Oncogene Proteins
  • Protein Serine-Threonine Kinases
  • Phosphorylation
  • Molecular Sequence Data