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Localization and structure of the ankyrin-binding site on beta2-spectrin.

Publication ,  Journal Article
Davis, L; Abdi, K; Machius, M; Brautigam, C; Tomchick, DR; Bennett, V; Michaely, P
Published in: J Biol Chem
March 13, 2009

Spectrins are tetrameric actin-cross-linking proteins that form an elastic network, termed the membrane skeleton, on the cytoplasmic surface of cellular membranes. At the plasma membrane, the membrane skeleton provides essential support, preventing loss of membrane material to environmental shear stresses. The skeleton also controls the location, abundance, and activity of membrane proteins that are critical to cell and tissue function. The ability of the skeleton to modulate membrane stability and function requires adaptor proteins that bind the skeleton to membranes. The principal adaptors are the ankyrin proteins, which bind to the beta-subunit of spectrin and to the cytoplasmic domains of numerous integral membrane proteins. Here, we present the crystal structure of the ankyrin-binding domain of human beta2-spectrin at 1.95 A resolution together with mutagenesis data identifying the binding surface for ankyrins on beta2-spectrin.

Duke Scholars

Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

March 13, 2009

Volume

284

Issue

11

Start / End Page

6982 / 6987

Location

United States

Related Subject Headings

  • Spectrin
  • Protein Structure, Tertiary
  • Peptide Mapping
  • Mutation, Missense
  • Humans
  • Cytoskeleton
  • Crystallography, X-Ray
  • Cell Membrane
  • Biochemistry & Molecular Biology
  • Binding Sites
 

Citation

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Davis, L., Abdi, K., Machius, M., Brautigam, C., Tomchick, D. R., Bennett, V., & Michaely, P. (2009). Localization and structure of the ankyrin-binding site on beta2-spectrin. J Biol Chem, 284(11), 6982–6987. https://doi.org/10.1074/jbc.M809245200
Davis, Lydia, Khadar Abdi, Mischa Machius, Chad Brautigam, Diana R. Tomchick, Vann Bennett, and Peter Michaely. “Localization and structure of the ankyrin-binding site on beta2-spectrin.J Biol Chem 284, no. 11 (March 13, 2009): 6982–87. https://doi.org/10.1074/jbc.M809245200.
Davis L, Abdi K, Machius M, Brautigam C, Tomchick DR, Bennett V, et al. Localization and structure of the ankyrin-binding site on beta2-spectrin. J Biol Chem. 2009 Mar 13;284(11):6982–7.
Davis, Lydia, et al. “Localization and structure of the ankyrin-binding site on beta2-spectrin.J Biol Chem, vol. 284, no. 11, Mar. 2009, pp. 6982–87. Pubmed, doi:10.1074/jbc.M809245200.
Davis L, Abdi K, Machius M, Brautigam C, Tomchick DR, Bennett V, Michaely P. Localization and structure of the ankyrin-binding site on beta2-spectrin. J Biol Chem. 2009 Mar 13;284(11):6982–6987.

Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

March 13, 2009

Volume

284

Issue

11

Start / End Page

6982 / 6987

Location

United States

Related Subject Headings

  • Spectrin
  • Protein Structure, Tertiary
  • Peptide Mapping
  • Mutation, Missense
  • Humans
  • Cytoskeleton
  • Crystallography, X-Ray
  • Cell Membrane
  • Biochemistry & Molecular Biology
  • Binding Sites