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Ankyrin-G regulates inactivation gating of the neuronal sodium channel, Nav1.6.

Publication ,  Journal Article
Shirahata, E; Iwasaki, H; Takagi, M; Lin, C; Bennett, V; Okamura, Y; Hayasaka, K
Published in: J Neurophysiol
September 2006

Ankyrin-G, a modular protein, plays a critical role in clustering voltage-gated sodium channels (Nav channels) in nodes of Ranvier and initial segments of mammalian neurons. However, direct effects of ankyrin-G on electrophysiological properties of Nav channels remain elusive. In this study, we explored whether ankyrin-G has a role in modifying gating properties of the neuronal Nav1.6 channel that is predominantly localized at nodes of Ranvier and initial segments. TsA201 cells transfected with the human Nav1.6 cDNA alone exhibited significant persistent sodium current (Ina-p). On the other hand, Ina-p was barely detected on co-expression with ankyrin-G. Ankyrin-B, another ankyrin, did not show such an effect. Expression of chimeras between the two isoforms of ankyrin suggests that the membrane-binding domain of ankyrin-G is critical for reducing the Ina-p of Nav1.6. These results suggest that ankyrin-G regulates neuronal excitability not only through clustering Nav channels but also by directly modifying their channel gating.

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Published In

J Neurophysiol

DOI

ISSN

0022-3077

Publication Date

September 2006

Volume

96

Issue

3

Start / End Page

1347 / 1357

Location

United States

Related Subject Headings

  • Tetrodotoxin
  • Spinal Cord
  • Sodium Channels
  • Reverse Transcriptase Polymerase Chain Reaction
  • Recombinant Fusion Proteins
  • Patch-Clamp Techniques
  • Neurons
  • Neurology & Neurosurgery
  • Nerve Tissue Proteins
  • NAV1.6 Voltage-Gated Sodium Channel
 

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Shirahata, E., Iwasaki, H., Takagi, M., Lin, C., Bennett, V., Okamura, Y., & Hayasaka, K. (2006). Ankyrin-G regulates inactivation gating of the neuronal sodium channel, Nav1.6. J Neurophysiol, 96(3), 1347–1357. https://doi.org/10.1152/jn.01264.2005
Shirahata, Emi, Hirohide Iwasaki, Masahiro Takagi, Changqing Lin, Vann Bennett, Yasushi Okamura, and Kiyoshi Hayasaka. “Ankyrin-G regulates inactivation gating of the neuronal sodium channel, Nav1.6.J Neurophysiol 96, no. 3 (September 2006): 1347–57. https://doi.org/10.1152/jn.01264.2005.
Shirahata E, Iwasaki H, Takagi M, Lin C, Bennett V, Okamura Y, et al. Ankyrin-G regulates inactivation gating of the neuronal sodium channel, Nav1.6. J Neurophysiol. 2006 Sep;96(3):1347–57.
Shirahata, Emi, et al. “Ankyrin-G regulates inactivation gating of the neuronal sodium channel, Nav1.6.J Neurophysiol, vol. 96, no. 3, Sept. 2006, pp. 1347–57. Pubmed, doi:10.1152/jn.01264.2005.
Shirahata E, Iwasaki H, Takagi M, Lin C, Bennett V, Okamura Y, Hayasaka K. Ankyrin-G regulates inactivation gating of the neuronal sodium channel, Nav1.6. J Neurophysiol. 2006 Sep;96(3):1347–1357.

Published In

J Neurophysiol

DOI

ISSN

0022-3077

Publication Date

September 2006

Volume

96

Issue

3

Start / End Page

1347 / 1357

Location

United States

Related Subject Headings

  • Tetrodotoxin
  • Spinal Cord
  • Sodium Channels
  • Reverse Transcriptase Polymerase Chain Reaction
  • Recombinant Fusion Proteins
  • Patch-Clamp Techniques
  • Neurons
  • Neurology & Neurosurgery
  • Nerve Tissue Proteins
  • NAV1.6 Voltage-Gated Sodium Channel