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Protein typing of circulating microvesicles allows real-time monitoring of glioblastoma therapy.

Publication ,  Journal Article
Shao, H; Chung, J; Balaj, L; Charest, A; Bigner, DD; Carter, BS; Hochberg, FH; Breakefield, XO; Weissleder, R; Lee, H
Published in: Nat Med
December 2012

Glioblastomas shed large quantities of small, membrane-bound microvesicles into the circulation. Although these hold promise as potential biomarkers of therapeutic response, their identification and quantification remain challenging. Here, we describe a highly sensitive and rapid analytical technique for profiling circulating microvesicles directly from blood samples of patients with glioblastoma. Microvesicles, introduced onto a dedicated microfluidic chip, are labeled with target-specific magnetic nanoparticles and detected by a miniaturized nuclear magnetic resonance system. Compared with current methods, this integrated system has a much higher detection sensitivity and can differentiate glioblastoma multiforme (GBM) microvesicles from nontumor host cell-derived microvesicles. We also show that circulating GBM microvesicles can be used to analyze primary tumor mutations and as a predictive metric of treatment-induced changes. This platform could provide both an early indicator of drug efficacy and a potential molecular stratifier for human clinical trials.

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Published In

Nat Med

DOI

EISSN

1546-170X

Publication Date

December 2012

Volume

18

Issue

12

Start / End Page

1835 / 1840

Location

United States

Related Subject Headings

  • Transport Vesicles
  • Microfluidic Analytical Techniques
  • Magnetite Nanoparticles
  • Magnetic Resonance Imaging
  • Immunology
  • Humans
  • Glioblastoma
  • Cell Line, Tumor
  • Biomarkers, Tumor
  • 42 Health sciences
 

Citation

APA
Chicago
ICMJE
MLA
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Shao, H., Chung, J., Balaj, L., Charest, A., Bigner, D. D., Carter, B. S., … Lee, H. (2012). Protein typing of circulating microvesicles allows real-time monitoring of glioblastoma therapy. Nat Med, 18(12), 1835–1840. https://doi.org/10.1038/nm.2994
Shao, Huilin, Jaehoon Chung, Leonora Balaj, Alain Charest, Darell D. Bigner, Bob S. Carter, Fred H. Hochberg, Xandra O. Breakefield, Ralph Weissleder, and Hakho Lee. “Protein typing of circulating microvesicles allows real-time monitoring of glioblastoma therapy.Nat Med 18, no. 12 (December 2012): 1835–40. https://doi.org/10.1038/nm.2994.
Shao H, Chung J, Balaj L, Charest A, Bigner DD, Carter BS, et al. Protein typing of circulating microvesicles allows real-time monitoring of glioblastoma therapy. Nat Med. 2012 Dec;18(12):1835–40.
Shao, Huilin, et al. “Protein typing of circulating microvesicles allows real-time monitoring of glioblastoma therapy.Nat Med, vol. 18, no. 12, Dec. 2012, pp. 1835–40. Pubmed, doi:10.1038/nm.2994.
Shao H, Chung J, Balaj L, Charest A, Bigner DD, Carter BS, Hochberg FH, Breakefield XO, Weissleder R, Lee H. Protein typing of circulating microvesicles allows real-time monitoring of glioblastoma therapy. Nat Med. 2012 Dec;18(12):1835–1840.

Published In

Nat Med

DOI

EISSN

1546-170X

Publication Date

December 2012

Volume

18

Issue

12

Start / End Page

1835 / 1840

Location

United States

Related Subject Headings

  • Transport Vesicles
  • Microfluidic Analytical Techniques
  • Magnetite Nanoparticles
  • Magnetic Resonance Imaging
  • Immunology
  • Humans
  • Glioblastoma
  • Cell Line, Tumor
  • Biomarkers, Tumor
  • 42 Health sciences