Skip to main content
Journal cover image

Functional differences in the multiple hemocyanins of the horseshoe crab, Limulus polyphemus L.

Publication ,  Journal Article
Sullivan, B; Bonaventura, J; Bonaventura, C
Published in: Proceedings of the National Academy of Sciences of the United States of America
June 1974

Hemocyanin in the hemolymph of the horseshoe crab, Limulus polyphemus L., is a high-molecular-weight copper protein which binds oxygen cooperatively and shows a higher oxygen affinity at pH 7 than at pH 9. Treatment with EDTA (ethylenediaminetetra-acetate) disaggregates the hemocyanin molecules and abolishes both the reverse Bohr effect and cooperative oxygen binding. Chloride ions interact with the EDTA-treated material and, in the presence of saturating amounts of NaCl, a reverse Bohr effect is restored, but cooperativity is not. The EDTA-treated hemocyanin contains at least five electrophoretically distinct hemocyanins. These hemocyanins have similar molecular weights (about 66,000) but are functionally dissimilar. They have different oxygen affinities and different responses to chloride ions. The effect of chloride ions on unfractionated hemocyanin is due to pH-dependent chloride interactions with only two of the five hemocyanin components. The functional differences between the hemocyanin components may provide Limulus with a valuable respiratory flexibility in its interaction with the environment. The kinetics of oxygen combination and dissociation for the various hemocyanin preparations show that variations in the rate of oxygen dissociation are primarily responsible for the observed differences in oxygen affinity. The rate of oxygen dissociation varies 20-fold under conditions where the apparent rate of oxygen combination shows less than a 2-fold variation. Cooperative interactions in the untreated hemocyanin are most obvious in the "off" reaction, which increases in rate as successive oxygen molecules are released.

Duke Scholars

Published In

Proceedings of the National Academy of Sciences of the United States of America

DOI

EISSN

1091-6490

ISSN

0027-8424

Publication Date

June 1974

Volume

71

Issue

6

Start / End Page

2558 / 2562

Related Subject Headings

  • Sodium Chloride
  • Protein Binding
  • Oxygen
  • Kinetics
  • Hydrogen-Ion Concentration
  • Hemolymph
  • Hemocyanins
  • Edetic Acid
  • Chromatography, Gel
  • Chlorides
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Sullivan, B., Bonaventura, J., & Bonaventura, C. (1974). Functional differences in the multiple hemocyanins of the horseshoe crab, Limulus polyphemus L. Proceedings of the National Academy of Sciences of the United States of America, 71(6), 2558–2562. https://doi.org/10.1073/pnas.71.6.2558
Sullivan, B., J. Bonaventura, and C. Bonaventura. “Functional differences in the multiple hemocyanins of the horseshoe crab, Limulus polyphemus L.Proceedings of the National Academy of Sciences of the United States of America 71, no. 6 (June 1974): 2558–62. https://doi.org/10.1073/pnas.71.6.2558.
Sullivan B, Bonaventura J, Bonaventura C. Functional differences in the multiple hemocyanins of the horseshoe crab, Limulus polyphemus L. Proceedings of the National Academy of Sciences of the United States of America. 1974 Jun;71(6):2558–62.
Sullivan, B., et al. “Functional differences in the multiple hemocyanins of the horseshoe crab, Limulus polyphemus L.Proceedings of the National Academy of Sciences of the United States of America, vol. 71, no. 6, June 1974, pp. 2558–62. Epmc, doi:10.1073/pnas.71.6.2558.
Sullivan B, Bonaventura J, Bonaventura C. Functional differences in the multiple hemocyanins of the horseshoe crab, Limulus polyphemus L. Proceedings of the National Academy of Sciences of the United States of America. 1974 Jun;71(6):2558–2562.
Journal cover image

Published In

Proceedings of the National Academy of Sciences of the United States of America

DOI

EISSN

1091-6490

ISSN

0027-8424

Publication Date

June 1974

Volume

71

Issue

6

Start / End Page

2558 / 2562

Related Subject Headings

  • Sodium Chloride
  • Protein Binding
  • Oxygen
  • Kinetics
  • Hydrogen-Ion Concentration
  • Hemolymph
  • Hemocyanins
  • Edetic Acid
  • Chromatography, Gel
  • Chlorides