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Identification of fluorescent beads using a coded aperture snapshot spectral imager.

Publication ,  Journal Article
Cull, CF; Choi, K; Brady, DJ; Oliver, T
Published in: Applied optics
April 2010

We apply a coded aperture snapshot spectral imager (CASSI) to fluorescence microscopy. CASSI records a two-dimensional (2D) spectrally filtered projection of a three-dimensional (3D) spectral data cube. We minimize a convex quadratic function with total variation (TV) constraints for data cube estimation from the 2D snapshot. We adapt the TV minimization algorithm for direct fluorescent bead identification from CASSI measurements by combining a priori knowledge of the spectra associated with each bead type. Our proposed method creates a 2D bead identity image. Simulated fluorescence CASSI measurements are used to evaluate the behavior of the algorithm. We also record real CASSI measurements of a ten bead type fluorescence scene and create a 2D bead identity map. A baseline image from filtered-array imaging system verifies CASSI's 2D bead identity map.

Duke Scholars

Published In

Applied optics

DOI

EISSN

1539-4522

ISSN

1559-128X

Publication Date

April 2010

Volume

49

Issue

10

Start / End Page

B59 / B70

Related Subject Headings

  • Spectrometry, Fluorescence
  • Optics
  • Optical Phenomena
  • Optical Devices
  • Microspheres
  • Imaging, Three-Dimensional
  • Fluorescent Dyes
  • Algorithms
  • 5102 Atomic, molecular and optical physics
  • 4008 Electrical engineering
 

Citation

APA
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ICMJE
MLA
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Cull, C. F., Choi, K., Brady, D. J., & Oliver, T. (2010). Identification of fluorescent beads using a coded aperture snapshot spectral imager. Applied Optics, 49(10), B59–B70. https://doi.org/10.1364/ao.49.000b59
Cull, Christy Fernandez, Kerkil Choi, David J. Brady, and Tim Oliver. “Identification of fluorescent beads using a coded aperture snapshot spectral imager.Applied Optics 49, no. 10 (April 2010): B59–70. https://doi.org/10.1364/ao.49.000b59.
Cull CF, Choi K, Brady DJ, Oliver T. Identification of fluorescent beads using a coded aperture snapshot spectral imager. Applied optics. 2010 Apr;49(10):B59–70.
Cull, Christy Fernandez, et al. “Identification of fluorescent beads using a coded aperture snapshot spectral imager.Applied Optics, vol. 49, no. 10, Apr. 2010, pp. B59–70. Epmc, doi:10.1364/ao.49.000b59.
Cull CF, Choi K, Brady DJ, Oliver T. Identification of fluorescent beads using a coded aperture snapshot spectral imager. Applied optics. 2010 Apr;49(10):B59–B70.

Published In

Applied optics

DOI

EISSN

1539-4522

ISSN

1559-128X

Publication Date

April 2010

Volume

49

Issue

10

Start / End Page

B59 / B70

Related Subject Headings

  • Spectrometry, Fluorescence
  • Optics
  • Optical Phenomena
  • Optical Devices
  • Microspheres
  • Imaging, Three-Dimensional
  • Fluorescent Dyes
  • Algorithms
  • 5102 Atomic, molecular and optical physics
  • 4008 Electrical engineering