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Characterization of the DNA- and metal-binding properties of Vibrio anguillarum fur reveals conservation of a structural Zn(2+) ion.

Publication ,  Journal Article
Zheleznova, EE; Crosa, JH; Brennan, RG
Published in: J Bacteriol
November 2000

The ferric uptake regulator, Fur, represses iron uptake and siderophore biosynthetic genes under iron-replete conditions. Here we report in vitro solution studies on Vibrio anguillarum Fur binding to the consensus 19-bp Escherichia coli iron box in the presence of several divalent metals. We found that V. anguillarum Fur binds the iron box in the presence of Mn(2+), Co(2+), Cd(2+), and to a lesser extent Ni(2+) but, unlike E. coli Fur, not in the presence of Zn(2+). We also found that V. anguillarum Fur contains a structural zinc ion that is necessary yet alone is insufficient for DNA binding.

Duke Scholars

Published In

J Bacteriol

DOI

ISSN

0021-9193

Publication Date

November 2000

Volume

182

Issue

21

Start / End Page

6264 / 6267

Location

United States

Related Subject Headings

  • Zinc
  • Vibrio
  • Sequence Alignment
  • Repressor Proteins
  • Protein Binding
  • Nickel
  • Molecular Sequence Data
  • Microbiology
  • Manganese
  • Genes, Bacterial
 

Citation

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ICMJE
MLA
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Zheleznova, E. E., Crosa, J. H., & Brennan, R. G. (2000). Characterization of the DNA- and metal-binding properties of Vibrio anguillarum fur reveals conservation of a structural Zn(2+) ion. J Bacteriol, 182(21), 6264–6267. https://doi.org/10.1128/JB.182.21.6264-6267.2000
Zheleznova, E. E., J. H. Crosa, and R. G. Brennan. “Characterization of the DNA- and metal-binding properties of Vibrio anguillarum fur reveals conservation of a structural Zn(2+) ion.J Bacteriol 182, no. 21 (November 2000): 6264–67. https://doi.org/10.1128/JB.182.21.6264-6267.2000.
Zheleznova, E. E., et al. “Characterization of the DNA- and metal-binding properties of Vibrio anguillarum fur reveals conservation of a structural Zn(2+) ion.J Bacteriol, vol. 182, no. 21, Nov. 2000, pp. 6264–67. Pubmed, doi:10.1128/JB.182.21.6264-6267.2000.

Published In

J Bacteriol

DOI

ISSN

0021-9193

Publication Date

November 2000

Volume

182

Issue

21

Start / End Page

6264 / 6267

Location

United States

Related Subject Headings

  • Zinc
  • Vibrio
  • Sequence Alignment
  • Repressor Proteins
  • Protein Binding
  • Nickel
  • Molecular Sequence Data
  • Microbiology
  • Manganese
  • Genes, Bacterial