Skip to main content
Journal cover image

Biphasic effects of interleukin-1beta on osteoblast differentiation in vitro.

Publication ,  Journal Article
Lin, F-H; Chang, JB; McGuire, MH; Yee, JA; Brigman, BE
Published in: J Orthop Res
July 2010

A rat calvarial cell model of osteoblast differentiation using the formation of bone nodules in vitro as an endpoint was used to assess the effects of IL-1beta on osteoblast differentiation. Short-term treatment (2 days) with IL-1beta early in culture resulted in increased nodule number and size as well as calcium content in contrast to long-term treatment (6 days) in cultures assessed at 10-12 days. This increase in bone formation was blocked by IL-1 receptor antagonists. Short-term treatment increased COX-2, prostaglandin (PGE(2)), and iNOS production. Exogenous PGE(2) with IL-1beta enhanced this effect. COX-2 inhibitors, indomethacin and N-39, blocked 50% of nodule formation. NO donor did not modify effects of IL-1beta, but iNOS inhibitor (1400W) partially blocked the effects. However, PGE(2) and NO donors could not rescue the decreased nodule number resulting from long-term IL-1beta treatment. The results of this study suggest a biphasic effect of IL-1beta on bone nodule formation activated by IL-1beta binding with IL-1 receptors, and the anabolic effect of early short-term treatment with IL-1beta is likely mediated by PGE without ruling out nitric oxide.

Duke Scholars

Published In

J Orthop Res

DOI

EISSN

1554-527X

Publication Date

July 2010

Volume

28

Issue

7

Start / End Page

958 / 964

Location

United States

Related Subject Headings

  • Skull
  • Receptors, Interleukin-1
  • Rats
  • Osteogenesis
  • Osteoblasts
  • Orthopedics
  • Nitric Oxide Synthase Type II
  • Nitric Oxide Donors
  • Interleukin-1beta
  • Indomethacin
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Lin, F.-H., Chang, J. B., McGuire, M. H., Yee, J. A., & Brigman, B. E. (2010). Biphasic effects of interleukin-1beta on osteoblast differentiation in vitro. J Orthop Res, 28(7), 958–964. https://doi.org/10.1002/jor.21099
Lin, Fu-Hsiumg, Jessica B. Chang, Michael H. McGuire, John A. Yee, and Brian E. Brigman. “Biphasic effects of interleukin-1beta on osteoblast differentiation in vitro.J Orthop Res 28, no. 7 (July 2010): 958–64. https://doi.org/10.1002/jor.21099.
Lin F-H, Chang JB, McGuire MH, Yee JA, Brigman BE. Biphasic effects of interleukin-1beta on osteoblast differentiation in vitro. J Orthop Res. 2010 Jul;28(7):958–64.
Lin, Fu-Hsiumg, et al. “Biphasic effects of interleukin-1beta on osteoblast differentiation in vitro.J Orthop Res, vol. 28, no. 7, July 2010, pp. 958–64. Pubmed, doi:10.1002/jor.21099.
Lin F-H, Chang JB, McGuire MH, Yee JA, Brigman BE. Biphasic effects of interleukin-1beta on osteoblast differentiation in vitro. J Orthop Res. 2010 Jul;28(7):958–964.
Journal cover image

Published In

J Orthop Res

DOI

EISSN

1554-527X

Publication Date

July 2010

Volume

28

Issue

7

Start / End Page

958 / 964

Location

United States

Related Subject Headings

  • Skull
  • Receptors, Interleukin-1
  • Rats
  • Osteogenesis
  • Osteoblasts
  • Orthopedics
  • Nitric Oxide Synthase Type II
  • Nitric Oxide Donors
  • Interleukin-1beta
  • Indomethacin