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Imaging synaptic inhibition in transgenic mice expressing the chloride indicator, Clomeleon.

Publication ,  Journal Article
Berglund, K; Schleich, W; Krieger, P; Loo, LS; Wang, D; Cant, NB; Feng, G; Augustine, GJ; Kuner, T
Published in: Brain Cell Biol
December 2006

We describe here a molecular genetic approach for imaging synaptic inhibition. The thy-1 promoter was used to express high levels of Clomeleon, a ratiometric fluorescent indicator for chloride ions, in discrete populations of neurons in the brains of transgenic mice. Clomeleon was functional after chronic expression and provided non-invasive readouts of intracellular chloride concentration ([Cl(-)](i)) in brain slices, allowing us to quantify age-dependent declines in resting [Cl(-)](i) during neuronal development. Activation of hippocampal interneurons caused [Cl(-)](i) to rise transiently in individual postsynaptic pyramidal neurons. [Cl(-)](i) increased in direct proportion to the amount of inhibitory transmission, with peak changes as large as 4 mM. Integrating responses over populations of pyramidal neurons allowed sensitive detection of synaptic inhibition. Thus, Clomeleon imaging permits non-invasive, spatiotemporally resolved recordings of [Cl(-)](i) in a large variety of neurons, opening up new opportunities for imaging synaptic inhibition and other forms of chloride signaling.

Duke Scholars

Published In

Brain Cell Biol

DOI

ISSN

1559-7105

Publication Date

December 2006

Volume

35

Issue

4-6

Start / End Page

207 / 228

Location

United States

Related Subject Headings

  • Synaptic Transmission
  • Synapses
  • Staining and Labeling
  • Recombinant Fusion Proteins
  • Pyramidal Cells
  • Patch-Clamp Techniques
  • Organ Culture Techniques
  • Neurophysiology
  • Neurology & Neurosurgery
  • Neurochemistry
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Berglund, K., Schleich, W., Krieger, P., Loo, L. S., Wang, D., Cant, N. B., … Kuner, T. (2006). Imaging synaptic inhibition in transgenic mice expressing the chloride indicator, Clomeleon. Brain Cell Biol, 35(4–6), 207–228. https://doi.org/10.1007/s11068-008-9019-6
Berglund, Ken, Wolfram Schleich, Patrik Krieger, Li Shen Loo, Dongqing Wang, Nell B. Cant, Guoping Feng, George J. Augustine, and Thomas Kuner. “Imaging synaptic inhibition in transgenic mice expressing the chloride indicator, Clomeleon.Brain Cell Biol 35, no. 4–6 (December 2006): 207–28. https://doi.org/10.1007/s11068-008-9019-6.
Berglund K, Schleich W, Krieger P, Loo LS, Wang D, Cant NB, et al. Imaging synaptic inhibition in transgenic mice expressing the chloride indicator, Clomeleon. Brain Cell Biol. 2006 Dec;35(4–6):207–28.
Berglund, Ken, et al. “Imaging synaptic inhibition in transgenic mice expressing the chloride indicator, Clomeleon.Brain Cell Biol, vol. 35, no. 4–6, Dec. 2006, pp. 207–28. Pubmed, doi:10.1007/s11068-008-9019-6.
Berglund K, Schleich W, Krieger P, Loo LS, Wang D, Cant NB, Feng G, Augustine GJ, Kuner T. Imaging synaptic inhibition in transgenic mice expressing the chloride indicator, Clomeleon. Brain Cell Biol. 2006 Dec;35(4–6):207–228.

Published In

Brain Cell Biol

DOI

ISSN

1559-7105

Publication Date

December 2006

Volume

35

Issue

4-6

Start / End Page

207 / 228

Location

United States

Related Subject Headings

  • Synaptic Transmission
  • Synapses
  • Staining and Labeling
  • Recombinant Fusion Proteins
  • Pyramidal Cells
  • Patch-Clamp Techniques
  • Organ Culture Techniques
  • Neurophysiology
  • Neurology & Neurosurgery
  • Neurochemistry