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β2-Adrenergic receptors in hamster smooth muscle cells are transcriptionally regulated by glucocorticoids

Publication ,  Journal Article
Collins, S; Caron, MG; Lefkowitz, RJ
Published in: Journal of Biological Chemistry
1988

Steroid hormones modulate adrenergic receptor responsiveness and receptor number. To investigate the regulation of the β2-adrenergic receptor gene by glucocorticoids we examined the effects of the synthetic glucocorticoid agonist triamcinolone acetonide on the expression of β2-adrenergic receptors in DDT1MF-2 hamster smooth muscle cells. Glucocorticoid treatment (1 x 10-7 M) produced a 2.2 ± 0.4-fold (n = 8) increase in β2-adrenergic receptor number (maximum between 6 and 12 h) as determined by radioligand binding and a similar increase in catecholamine-stimulated adenylate cyclase activity. Steady-state levels of β2-adrenergic receptor mRNA, analyzed by Northern blot hybridization, were increased 2.4 ± 0.4-fold (n = 6) within 1 h, while actin mRNA levels were unchanged throughout the experiment. These steroid-induced increases in β2-adrenergic receptor mRNA returned to control levels by 24 h and were followed by a much slower decline in β2-adrenergic receptor in plasma membranes. The rate of β2-adrenergic receptor gene transcription, assessed by nuclear run-off transcription assays, increased 3.1 ± 0.1-fold (n = 2) in cells treated for 30 min with 1 x 10-7 M triamcinolone acetonide. These studies indicate that glucocorticoids regulate the β2-adrenergic receptor-adenylate cyclase system by controlling the rate of transcription of the β2-adrenergic receptor gene and hence the responsiveness of the enzyme to catecholamine stimulation.

Duke Scholars

Published In

Journal of Biological Chemistry

Publication Date

1988

Volume

263

Issue

19

Start / End Page

9067 / 9070

Related Subject Headings

  • Biochemistry & Molecular Biology
  • 34 Chemical sciences
  • 32 Biomedical and clinical sciences
  • 31 Biological sciences
  • 11 Medical and Health Sciences
  • 06 Biological Sciences
  • 03 Chemical Sciences
 

Citation

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Collins, S., Caron, M. G., & Lefkowitz, R. J. (1988). β2-Adrenergic receptors in hamster smooth muscle cells are transcriptionally regulated by glucocorticoids. Journal of Biological Chemistry, 263(19), 9067–9070.
Collins, S., M. G. Caron, and R. J. Lefkowitz. “β2-Adrenergic receptors in hamster smooth muscle cells are transcriptionally regulated by glucocorticoids.” Journal of Biological Chemistry 263, no. 19 (1988): 9067–70.
Collins S, Caron MG, Lefkowitz RJ. β2-Adrenergic receptors in hamster smooth muscle cells are transcriptionally regulated by glucocorticoids. Journal of Biological Chemistry. 1988;263(19):9067–70.
Collins, S., et al. “β2-Adrenergic receptors in hamster smooth muscle cells are transcriptionally regulated by glucocorticoids.” Journal of Biological Chemistry, vol. 263, no. 19, 1988, pp. 9067–70.
Collins S, Caron MG, Lefkowitz RJ. β2-Adrenergic receptors in hamster smooth muscle cells are transcriptionally regulated by glucocorticoids. Journal of Biological Chemistry. 1988;263(19):9067–9070.

Published In

Journal of Biological Chemistry

Publication Date

1988

Volume

263

Issue

19

Start / End Page

9067 / 9070

Related Subject Headings

  • Biochemistry & Molecular Biology
  • 34 Chemical sciences
  • 32 Biomedical and clinical sciences
  • 31 Biological sciences
  • 11 Medical and Health Sciences
  • 06 Biological Sciences
  • 03 Chemical Sciences