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Insulin-like growth factor 1 mitigates hematopoietic toxicity after lethal total body irradiation.

Publication ,  Journal Article
Zhou, D; Deoliveira, D; Kang, Y; Choi, SS; Li, Z; Chao, NJ; Chen, BJ
Published in: Int J Radiat Oncol Biol Phys
March 15, 2013

PURPOSE: To investigate whether and how insulin-like growth factor 1 (IGF-1) mitigates hematopoietic toxicity after total body irradiation. METHODS AND MATERIALS: BALB/c mice were irradiated with a lethal dose of radiation (7.5 Gy) and treated with IGF-1 at a dose of 100 μg/dose intravenously once a day for 5 consecutive days starting within 1 hour after exposure. Survival and hematopoietic recovery were monitored. The mechanisms by which IGF-1 promotes hematopoietic recovery were also studied by use of an in vitro culture system. RESULTS: IGF-1 protected 8 of 20 mice (40%) from lethal irradiation, whereas only 2 of 20 mice (10%) in the saline control group survived for more than 100 days after irradiation. A single dose of IGF-1 (500 μg) was as effective as daily dosing for 5 days. Positive effects were noted even when the initiation of treatment was delayed as long as 6 hours after irradiation. In comparison with the saline control group, treatment with IGF-1 significantly accelerated the recovery of both platelets and red blood cells in peripheral blood, total cell numbers, hematopoietic stem cells, and progenitor cells in the bone marrow when measured at day 14 after irradiation. IGF-1 protected both hematopoietic stem cells and progenitor cells from radiation-induced apoptosis and cell death. In addition, IGF-1 was able to facilitate the proliferation and differentiation of nonirradiated and irradiated hematopoietic progenitor cells. CONCLUSIONS: IGF-1 mitigates radiation-induced hematopoietic toxicity through protecting hematopoietic stem cells and progenitor cells from apoptosis and enhancing proliferation and differentiation of the surviving hematopoietic progenitor cells.

Duke Scholars

Published In

Int J Radiat Oncol Biol Phys

DOI

EISSN

1879-355X

Publication Date

March 15, 2013

Volume

85

Issue

4

Start / End Page

1141 / 1148

Location

United States

Related Subject Headings

  • Whole-Body Irradiation
  • Time Factors
  • Radiation Dosage
  • Oncology & Carcinogenesis
  • Mice, Inbred C57BL
  • Mice, Inbred BALB C
  • Mice
  • Insulin-Like Growth Factor I
  • Hematopoietic Stem Cells
  • Hematopoiesis
 

Citation

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MLA
NLM
Zhou, D., Deoliveira, D., Kang, Y., Choi, S. S., Li, Z., Chao, N. J., & Chen, B. J. (2013). Insulin-like growth factor 1 mitigates hematopoietic toxicity after lethal total body irradiation. Int J Radiat Oncol Biol Phys, 85(4), 1141–1148. https://doi.org/10.1016/j.ijrobp.2012.08.014
Zhou, Dunhua, Divino Deoliveira, Yubin Kang, Seung S. Choi, Zhiguo Li, Nelson J. Chao, and Benny J. Chen. “Insulin-like growth factor 1 mitigates hematopoietic toxicity after lethal total body irradiation.Int J Radiat Oncol Biol Phys 85, no. 4 (March 15, 2013): 1141–48. https://doi.org/10.1016/j.ijrobp.2012.08.014.
Zhou D, Deoliveira D, Kang Y, Choi SS, Li Z, Chao NJ, et al. Insulin-like growth factor 1 mitigates hematopoietic toxicity after lethal total body irradiation. Int J Radiat Oncol Biol Phys. 2013 Mar 15;85(4):1141–8.
Zhou, Dunhua, et al. “Insulin-like growth factor 1 mitigates hematopoietic toxicity after lethal total body irradiation.Int J Radiat Oncol Biol Phys, vol. 85, no. 4, Mar. 2013, pp. 1141–48. Pubmed, doi:10.1016/j.ijrobp.2012.08.014.
Zhou D, Deoliveira D, Kang Y, Choi SS, Li Z, Chao NJ, Chen BJ. Insulin-like growth factor 1 mitigates hematopoietic toxicity after lethal total body irradiation. Int J Radiat Oncol Biol Phys. 2013 Mar 15;85(4):1141–1148.
Journal cover image

Published In

Int J Radiat Oncol Biol Phys

DOI

EISSN

1879-355X

Publication Date

March 15, 2013

Volume

85

Issue

4

Start / End Page

1141 / 1148

Location

United States

Related Subject Headings

  • Whole-Body Irradiation
  • Time Factors
  • Radiation Dosage
  • Oncology & Carcinogenesis
  • Mice, Inbred C57BL
  • Mice, Inbred BALB C
  • Mice
  • Insulin-Like Growth Factor I
  • Hematopoietic Stem Cells
  • Hematopoiesis