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Glycogen debranching enzyme is associated with rat skeletal muscle sarcoplasmic reticulum.

Publication ,  Journal Article
Lees, SJ; Chen, Y-T; Williams, JH
Published in: Acta Physiol Scand
June 2004

AIMS: Gel electrophoresis revealed a band of molecular weight approximately 160 000 Da associated with the skeletal muscle sarcoplasmic reticulum (SR) vesicle preparations. This investigation sought to examine glycogen debranching enzyme associated with skeletal muscle SR. METHODS: Sarcoplasmic reticulum samples were also taken from muscle whose glycogen content had been reduced either via stimulation of the sciatic nerve or alpha-amylase treatment of muscle homogenates. RESULTS: The stimulation protocol reduced whole muscle glycogen by 86% (7.4 +/- 0.4 vs. 1.0 +/- 0.3 microg mg(-1) wet mass, P < or = 0.05). Glycogen associated with the SR was reduced by 82% in the stimulation protocol (533 +/- 82 vs. 96 +/- 7 microg mg(-1) protein) and by 94% in alpha-amylase treatment (493 +/- 11 vs. 29 +/- 2 microg mg(-1) protein), respectively. Gel electrophoresis and Western blots revealed that the content of glycogen debranching enzyme was reduced by approximately 53% as a result of muscle stimulation and by approximately 46% in alpha-amylase treatment (P < or = 0.05). In addition, glycogen debranching enzyme activity was reduced by 61% in stimulated samples compared with control (20.3 +/- 1.0 vs. 8.0 +/- 1.2 nmol mg(-1) min(-1), respectively), a value consistent with reductions observed from gel electrophoresis and Western blots. CONCLUSION: These results confirm that similar to glycogen phosphorylase, glycogen debranching enzyme is associated with the skeletal muscle SR and is dissociated under exercise conditions.

Duke Scholars

Published In

Acta Physiol Scand

DOI

ISSN

0001-6772

Publication Date

June 2004

Volume

181

Issue

2

Start / End Page

239 / 245

Location

England

Related Subject Headings

  • Sciatic Nerve
  • Sarcoplasmic Reticulum
  • Rats, Sprague-Dawley
  • Rats
  • Physiology
  • Physical Conditioning, Animal
  • Muscle, Skeletal
  • Glycogen Phosphorylase
  • Glycogen Debranching Enzyme System
  • Glycogen
 

Citation

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Lees, S. J., Chen, Y.-T., & Williams, J. H. (2004). Glycogen debranching enzyme is associated with rat skeletal muscle sarcoplasmic reticulum. Acta Physiol Scand, 181(2), 239–245. https://doi.org/10.1111/j.1365-201X.2004.01278.x
Lees, S. J., Y. -. T. Chen, and J. H. Williams. “Glycogen debranching enzyme is associated with rat skeletal muscle sarcoplasmic reticulum.Acta Physiol Scand 181, no. 2 (June 2004): 239–45. https://doi.org/10.1111/j.1365-201X.2004.01278.x.
Lees SJ, Chen Y-T, Williams JH. Glycogen debranching enzyme is associated with rat skeletal muscle sarcoplasmic reticulum. Acta Physiol Scand. 2004 Jun;181(2):239–45.
Lees, S. J., et al. “Glycogen debranching enzyme is associated with rat skeletal muscle sarcoplasmic reticulum.Acta Physiol Scand, vol. 181, no. 2, June 2004, pp. 239–45. Pubmed, doi:10.1111/j.1365-201X.2004.01278.x.
Lees SJ, Chen Y-T, Williams JH. Glycogen debranching enzyme is associated with rat skeletal muscle sarcoplasmic reticulum. Acta Physiol Scand. 2004 Jun;181(2):239–245.

Published In

Acta Physiol Scand

DOI

ISSN

0001-6772

Publication Date

June 2004

Volume

181

Issue

2

Start / End Page

239 / 245

Location

England

Related Subject Headings

  • Sciatic Nerve
  • Sarcoplasmic Reticulum
  • Rats, Sprague-Dawley
  • Rats
  • Physiology
  • Physical Conditioning, Animal
  • Muscle, Skeletal
  • Glycogen Phosphorylase
  • Glycogen Debranching Enzyme System
  • Glycogen