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In situ hybridization detection of estradiol-induced changes in ribosomal RNA levels in rat brain.

Publication ,  Journal Article
Jones, KJ; Chikaraishi, DM; Harrington, CA; McEwen, BS; Pfaff, DW
Published in: Brain Res
November 1986

In this study, quantitative assessment of estradiol (E2)-induced changes in levels of ribosomal RNA within brain regions concentrating the hormone was accomplished by in situ hybridization with nick-translated tritiated ribosomal DNA probes and use of a computer-based image analysis system. Ovariectomized rats were either implanted with estradiol capsules for 6 h, 24 h, or 15 days, or sham-implanted under the same time course to serve as controls. The mean number of grains, somal area, and grain density of neurons within three E2-concentrating brain regions, the ventrolateral portion of the ventromedial and the arcuate nuclei of the hypothalamus (VL-VMN and ARC, respectively) and the corticomedial nucleus of the amygdala (AMY) were determined. In the VL-VMN and ARC, levels of rRNA were significantly increased after 6 h of E2 treatment (70% and 30%, respectively) and after 24 h of E2 treatment (60% and 62%, respectively). However, these effects on rRNA levels in VL-VMN and ARC were not observed after prolonged exposure of 15 days to the hormone. Neuronal hypertrophy was present only after 24 h of E2 treatment in the VL-VMN and ARC (32% and 14%, respectively). No changes were found in the AMY. As an additional internal control, measurements were also collected from the dorsomedial portion of the VMN (DM-VMN), a region with few E2-concentrating neurons. No changes in any of the parameters were found in DM-VMN at any time after exposure to the hormone. By extending the in situ hybridization technique to the quantitative level, these findings demonstrate differential estrogenic regulation of a known gene product, rRNA, in rat brain that is temporally and regionally specific.

Duke Scholars

Published In

Brain Res

DOI

ISSN

0006-8993

Publication Date

November 1986

Volume

387

Issue

2

Start / End Page

145 / 152

Location

Netherlands

Related Subject Headings

  • Rats
  • RNA, Ribosomal
  • Nucleic Acid Hybridization
  • Neurons
  • Neurology & Neurosurgery
  • Limbic System
  • Image Processing, Computer-Assisted
  • Female
  • Estradiol
  • DNA, Ribosomal
 

Citation

APA
Chicago
ICMJE
MLA
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Jones, K. J., Chikaraishi, D. M., Harrington, C. A., McEwen, B. S., & Pfaff, D. W. (1986). In situ hybridization detection of estradiol-induced changes in ribosomal RNA levels in rat brain. Brain Res, 387(2), 145–152. https://doi.org/10.1016/0169-328x(86)90006-9
Jones, K. J., D. M. Chikaraishi, C. A. Harrington, B. S. McEwen, and D. W. Pfaff. “In situ hybridization detection of estradiol-induced changes in ribosomal RNA levels in rat brain.Brain Res 387, no. 2 (November 1986): 145–52. https://doi.org/10.1016/0169-328x(86)90006-9.
Jones KJ, Chikaraishi DM, Harrington CA, McEwen BS, Pfaff DW. In situ hybridization detection of estradiol-induced changes in ribosomal RNA levels in rat brain. Brain Res. 1986 Nov;387(2):145–52.
Jones, K. J., et al. “In situ hybridization detection of estradiol-induced changes in ribosomal RNA levels in rat brain.Brain Res, vol. 387, no. 2, Nov. 1986, pp. 145–52. Pubmed, doi:10.1016/0169-328x(86)90006-9.
Jones KJ, Chikaraishi DM, Harrington CA, McEwen BS, Pfaff DW. In situ hybridization detection of estradiol-induced changes in ribosomal RNA levels in rat brain. Brain Res. 1986 Nov;387(2):145–152.
Journal cover image

Published In

Brain Res

DOI

ISSN

0006-8993

Publication Date

November 1986

Volume

387

Issue

2

Start / End Page

145 / 152

Location

Netherlands

Related Subject Headings

  • Rats
  • RNA, Ribosomal
  • Nucleic Acid Hybridization
  • Neurons
  • Neurology & Neurosurgery
  • Limbic System
  • Image Processing, Computer-Assisted
  • Female
  • Estradiol
  • DNA, Ribosomal