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Active immunotherapy with transiently transfected cytokine-secreting tumor cells inhibits breast cancer metastases in tumor-bearing animals.

Publication ,  Journal Article
Coveney, E; Clary, B; Iacobucci, M; Philip, R; Lyerly, K
Published in: Surgery
August 1996

BACKGROUND: Metastatic disease remains the most frequent cause of treatment failure in the management of patients with breast cancer. A novel method that allows delivery of a gene into primary tumor cells was used to generate tumor cell vaccines to inhibit metastasis formation in tumor-bearing hosts. METHODS: Inoculation of 2.5 x 10(4) 4T1 murine breast cancer cells into the footpads of BALB/c mice reliably leads to tumor growth and pulmonary metastases. Interleukin-2 (IL-2)-secreting 4T1 cells (4T1-pMP6A/IL-2) and control transduced 4T1 cells (4T1-pMP6A) were generated by lipofection with a cationic liposome complexed to an adeno-associated viral plasmid bearing the IL-2 gene (pMP6A/IL-2). Unmodified 4T1 cells were inoculated into the footpads on day 0, and weekly immunization with phosphate-buffered saline solution or 2 x 10(6) irradiated 4T1, 4T1-pMP6A, or 4T1-pMP6A/IL-2 cells commenced on day 21. Hindlimb amputation was performed when tumors measured 6 mm in diameter. Mice were killed 24 days after amputation, and metastatic disease was determined by weighing lungs at time of harvest. RESULTS: A significant reduction was seen in the pulmonary metastatic load of mice receiving IL-2 gene-modified tumor cell immunization (4T1-pMP6A/IL2) when compared with mice given control immunizations. CONCLUSIONS: These results suggest that active immunization strategies with cytokine gene-modified tumor cells generated by clinically relevant gene delivery systems may prove useful in inhibiting the development of metastases from primary breast cancer.

Duke Scholars

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Published In

Surgery

DOI

ISSN

0039-6060

Publication Date

August 1996

Volume

120

Issue

2

Start / End Page

265 / 272

Location

United States

Related Subject Headings

  • Vaccines
  • Tumor Cells, Cultured
  • Transfection
  • Time Factors
  • Surgery
  • Plasmids
  • Mice, Inbred BALB C
  • Mice
  • Mammary Neoplasms, Experimental
  • Male
 

Citation

APA
Chicago
ICMJE
MLA
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Coveney, E., Clary, B., Iacobucci, M., Philip, R., & Lyerly, K. (1996). Active immunotherapy with transiently transfected cytokine-secreting tumor cells inhibits breast cancer metastases in tumor-bearing animals. Surgery, 120(2), 265–272. https://doi.org/10.1016/s0039-6060(96)80297-2
Coveney, E., B. Clary, M. Iacobucci, R. Philip, and K. Lyerly. “Active immunotherapy with transiently transfected cytokine-secreting tumor cells inhibits breast cancer metastases in tumor-bearing animals.Surgery 120, no. 2 (August 1996): 265–72. https://doi.org/10.1016/s0039-6060(96)80297-2.
Coveney, E., et al. “Active immunotherapy with transiently transfected cytokine-secreting tumor cells inhibits breast cancer metastases in tumor-bearing animals.Surgery, vol. 120, no. 2, Aug. 1996, pp. 265–72. Pubmed, doi:10.1016/s0039-6060(96)80297-2.
Journal cover image

Published In

Surgery

DOI

ISSN

0039-6060

Publication Date

August 1996

Volume

120

Issue

2

Start / End Page

265 / 272

Location

United States

Related Subject Headings

  • Vaccines
  • Tumor Cells, Cultured
  • Transfection
  • Time Factors
  • Surgery
  • Plasmids
  • Mice, Inbred BALB C
  • Mice
  • Mammary Neoplasms, Experimental
  • Male