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The induction of nitric oxide by polyribonucleotides in hamster and human glia

Publication ,  Journal Article
Snell, J; Chemvshev, O; Gilbert, D; Colton, C
Published in: FASEB Journal
December 1, 1996

Nitric oxide (NO), production by microglia and astrocytes is well characterized in cells isolated from rat or mouse CNS. However, notable differences in the levels of NO generated from both hamster and human glia have led us to examine the induction of nitric oxide synthase in these species. In contrast to the high-output pathway of NO production from rat/mouse glia in response to several inflammatory and immune mediators, hamster/human glia respond with high-output NO only under specific conditions such as treatment with polyribonucleoddes or the ingestion of foreign material. For these studies, hamster or human microglia and astrocytes were either separated or used as a mixed glial culture and plated into multiwell dishes. Cells were then continually stimulated with polyribonucleotides or other mediators, such as concanavalin A, for several days and the supernatants from each well were assayed for nitrite using the Griess assay. Hamster and human microglia could be induced to produce high levels of nitrite when the cells were stimulated with agents such as poly I:poly C. The data suggest that while cytokine stimulation of hamster or human glia is generally ineffective in inducing NO, agents associated with certain membrane receptors can induce high-output NO production.

Duke Scholars

Published In

FASEB Journal

ISSN

0892-6638

Publication Date

December 1, 1996

Volume

10

Issue

3

Related Subject Headings

  • Biochemistry & Molecular Biology
  • 1116 Medical Physiology
  • 0606 Physiology
  • 0601 Biochemistry and Cell Biology
 

Citation

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Snell, J., Chemvshev, O., Gilbert, D., & Colton, C. (1996). The induction of nitric oxide by polyribonucleotides in hamster and human glia. FASEB Journal, 10(3).
Snell, J., O. Chemvshev, D. Gilbert, and C. Colton. “The induction of nitric oxide by polyribonucleotides in hamster and human glia.” FASEB Journal 10, no. 3 (December 1, 1996).
Snell J, Chemvshev O, Gilbert D, Colton C. The induction of nitric oxide by polyribonucleotides in hamster and human glia. FASEB Journal. 1996 Dec 1;10(3).
Snell, J., et al. “The induction of nitric oxide by polyribonucleotides in hamster and human glia.” FASEB Journal, vol. 10, no. 3, Dec. 1996.
Snell J, Chemvshev O, Gilbert D, Colton C. The induction of nitric oxide by polyribonucleotides in hamster and human glia. FASEB Journal. 1996 Dec 1;10(3).

Published In

FASEB Journal

ISSN

0892-6638

Publication Date

December 1, 1996

Volume

10

Issue

3

Related Subject Headings

  • Biochemistry & Molecular Biology
  • 1116 Medical Physiology
  • 0606 Physiology
  • 0601 Biochemistry and Cell Biology