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(Pro)renin receptor is expressed in human retinal pigment epithelium and participates in extracellular matrix remodeling.

Publication ,  Journal Article
Alcazar, O; Cousins, SW; Striker, GE; Marin-Castano, ME
Published in: Exp Eye Res
November 2009

The (pro)renin receptor (PRR) is believed to potentiate the renin-angiotensin system (RAS), conferring to prorenin, a likely pathological role at tissue level. The PRR has been identified in the microvascular endothelial cells of the retina, in which it seems to be involved in pathological neovascularization processes. In the present study, we sought to explore PRR expression and prorenin action in human retinal pigment epithelium (RPE) cells, as well as its potential implication in extracellular matrix (ECM) turnover. Isolated RPE cells from donor human eyes as well as freshly isolated human retinas demonstrated expression of PRR at mRNA and protein levels. Moreover, we demonstrate that PRR expressed in the RPE cells is functional, as shown by prorenin-induced increases in Erk1/2 phosphorylation. PRR expression was also shown to be regulated by its main physiological agonist prorenin. We found evidence that the PRR may be involved in ECM-remodeling processes through a prorenin-induced upregulation of type I collagen. Immunostaining analysis of human retinas revealed higher PRR and type I collagen expression in the RPE of eye donors with dry age-related macular degeneration (AMD) and hypertension, supporting the in vitro findings using human-isolated RPE cells. Taken together, the present study demonstrates for the first time that the PRR is expressed in human RPE and suggests a molecular mechanism by which hypertension may exacerbate the pathology of dry AMD.

Duke Scholars

Published In

Exp Eye Res

DOI

EISSN

1096-0007

Publication Date

November 2009

Volume

89

Issue

5

Start / End Page

638 / 647

Location

England

Related Subject Headings

  • Up-Regulation
  • Transcription, Genetic
  • Retinal Pigment Epithelium
  • Renin
  • Receptors, Cell Surface
  • RNA, Messenger
  • Prorenin Receptor
  • Phosphorylation
  • Ophthalmology & Optometry
  • Mitogen-Activated Protein Kinase 3
 

Citation

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Alcazar, O., Cousins, S. W., Striker, G. E., & Marin-Castano, M. E. (2009). (Pro)renin receptor is expressed in human retinal pigment epithelium and participates in extracellular matrix remodeling. Exp Eye Res, 89(5), 638–647. https://doi.org/10.1016/j.exer.2009.06.014
Alcazar, Oscar, Scott W. Cousins, Gary E. Striker, and Maria E. Marin-Castano. “(Pro)renin receptor is expressed in human retinal pigment epithelium and participates in extracellular matrix remodeling.Exp Eye Res 89, no. 5 (November 2009): 638–47. https://doi.org/10.1016/j.exer.2009.06.014.
Alcazar O, Cousins SW, Striker GE, Marin-Castano ME. (Pro)renin receptor is expressed in human retinal pigment epithelium and participates in extracellular matrix remodeling. Exp Eye Res. 2009 Nov;89(5):638–47.
Alcazar, Oscar, et al. “(Pro)renin receptor is expressed in human retinal pigment epithelium and participates in extracellular matrix remodeling.Exp Eye Res, vol. 89, no. 5, Nov. 2009, pp. 638–47. Pubmed, doi:10.1016/j.exer.2009.06.014.
Alcazar O, Cousins SW, Striker GE, Marin-Castano ME. (Pro)renin receptor is expressed in human retinal pigment epithelium and participates in extracellular matrix remodeling. Exp Eye Res. 2009 Nov;89(5):638–647.
Journal cover image

Published In

Exp Eye Res

DOI

EISSN

1096-0007

Publication Date

November 2009

Volume

89

Issue

5

Start / End Page

638 / 647

Location

England

Related Subject Headings

  • Up-Regulation
  • Transcription, Genetic
  • Retinal Pigment Epithelium
  • Renin
  • Receptors, Cell Surface
  • RNA, Messenger
  • Prorenin Receptor
  • Phosphorylation
  • Ophthalmology & Optometry
  • Mitogen-Activated Protein Kinase 3