Skip to main content

Late outgrowth endothelial progenitor cells in patients with age-related macular degeneration.

Publication ,  Journal Article
Thill, M; Strunnikova, NV; Berna, MJ; Gordiyenko, N; Schmid, K; Cousins, SW; Thompson, DJS; Csaky, KG
Published in: Invest Ophthalmol Vis Sci
June 2008

PURPOSE: To evaluate the feasibility of isolating and expanding endothelial progenitor cells (EPCs), in the form of late outgrowth endothelial progenitor cells (OECs), from the peripheral blood of an aged population, particularly patients affected by different forms of AMD. METHODS: Peripheral blood mononuclear cells were collected from young control subjects (n = 18) and from elderly subjects with non-AMD/low-risk dry AMD (n = 15), high-risk dry AMD (n = 6), or neovascular AMD (nvAMD; n = 32); cultured in established conditions; and observed for appearance of OEC clusters and growth characteristics on expansion. Expression of VEGF receptor-2 (KDR) in OECs after expansion was determined by Western blot. Plasma samples of study subjects were analyzed for CRP and VEGF levels. RESULTS: OEC cultures were successfully generated from a similar number of subjects in each group. After adjustment for all other variables, subjects with high-risk dry AMD had a 5.6-fold higher number of OEC clusters per 20 mL blood, and subjects with nvAMD had a 5.1-fold high number than did subjects with non-AMD/low-risk dry AMD (P < 0.05). High-risk dry AMD generated 63 times more (NS) and nvAMD 32-times more (P < 0.05) OECs on expansion of clusters than did non-AMD/low-risk dry AMD. Population doubling occurred significantly faster in cultures from nvAMD eyes compared to non-AMD/low-risk dry AMD eyes. In addition, a significant correlation between the number of OEC clusters, expanded OECs and levels of KDR was demonstrated. CONCLUSIONS: An OEC population was isolated and expanded from the blood of elderly control and AMD-affected patients and demonstrated significantly higher number of initial OEC clusters and expansion potential of OECs in patients at risk for or already affected by nvAMD. OECs may be used for further phenotypic, genetic, and functional analyses in patients with nvAMD.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Invest Ophthalmol Vis Sci

DOI

ISSN

0146-0404

Publication Date

June 2008

Volume

49

Issue

6

Start / End Page

2696 / 2708

Location

United States

Related Subject Headings

  • Vascular Endothelial Growth Factor Receptor-2
  • Vascular Endothelial Growth Factor A
  • Stem Cells
  • Retinal Vessels
  • Ophthalmology & Optometry
  • Middle Aged
  • Male
  • Macular Degeneration
  • Immunophenotyping
  • Humans
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Thill, M., Strunnikova, N. V., Berna, M. J., Gordiyenko, N., Schmid, K., Cousins, S. W., … Csaky, K. G. (2008). Late outgrowth endothelial progenitor cells in patients with age-related macular degeneration. Invest Ophthalmol Vis Sci, 49(6), 2696–2708. https://doi.org/10.1167/iovs.07-0955
Thill, Michelle, Natalya V. Strunnikova, Marc J. Berna, Nataliya Gordiyenko, Kristin Schmid, Scott W. Cousins, Darby J. S. Thompson, and Karl G. Csaky. “Late outgrowth endothelial progenitor cells in patients with age-related macular degeneration.Invest Ophthalmol Vis Sci 49, no. 6 (June 2008): 2696–2708. https://doi.org/10.1167/iovs.07-0955.
Thill M, Strunnikova NV, Berna MJ, Gordiyenko N, Schmid K, Cousins SW, et al. Late outgrowth endothelial progenitor cells in patients with age-related macular degeneration. Invest Ophthalmol Vis Sci. 2008 Jun;49(6):2696–708.
Thill, Michelle, et al. “Late outgrowth endothelial progenitor cells in patients with age-related macular degeneration.Invest Ophthalmol Vis Sci, vol. 49, no. 6, June 2008, pp. 2696–708. Pubmed, doi:10.1167/iovs.07-0955.
Thill M, Strunnikova NV, Berna MJ, Gordiyenko N, Schmid K, Cousins SW, Thompson DJS, Csaky KG. Late outgrowth endothelial progenitor cells in patients with age-related macular degeneration. Invest Ophthalmol Vis Sci. 2008 Jun;49(6):2696–2708.

Published In

Invest Ophthalmol Vis Sci

DOI

ISSN

0146-0404

Publication Date

June 2008

Volume

49

Issue

6

Start / End Page

2696 / 2708

Location

United States

Related Subject Headings

  • Vascular Endothelial Growth Factor Receptor-2
  • Vascular Endothelial Growth Factor A
  • Stem Cells
  • Retinal Vessels
  • Ophthalmology & Optometry
  • Middle Aged
  • Male
  • Macular Degeneration
  • Immunophenotyping
  • Humans