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Wasting and macrophage production of tumor necrosis factor/cachectin and interleukin 1 in experimental visceral leishmaniasis.

Publication ,  Journal Article
Pearson, RD; Cox, G; Evans, T; Smith, DL; Weidel, D; Castracane, J
Published in: Am J Trop Med Hyg
December 1990

Wasting and secretion of the catabolic cytokines tumor necrosis factor (TNF)/cachectin and interleukin 1 (IL-1) were assessed in weanling Syrian hamsters infected with Leishmania donovani amastigotes. Whereas the mean weight of uninfected animals increased progressively over 9 weeks, the mean weight of infected animals plateaued at 4-6 weeks and then decreased progressively until death. Splenic mononuclear cells from control hamsters produced 11.3 +/- 8.3 (SD) ng TNF/10(6) mononuclear cells/24 hr. TNF secretion in infected animals was greater than the mean +/- 2 SD of controls in 1 of 3 hamsters at 2 weeks post-infection and in 8 of 9 hamsters at weeks 4-8. The mean TNF secreted by infected animals studied at weeks 4-8 was 371 (range 28-800) ng TNF/10(6) mononuclear cells/24 hr (P = 0.005). Control hamsters produced 7.7 +/- 2.7 pg IL-1/10(6) mononuclear cells/24 hr. At 2 weeks, mononuclear cells from 2 of 3 infected animals secreted amounts of IL-1 greater than the mean +/- 2 SD of controls. All of 8 infected hamsters secreted increased amounts of IL-1 at 4-8 weeks. The mean was 164 (range 17-370) pg IL-1/10(6) mononuclear cells/24 hr (P = 0.002). In comparison to infected animals, mononuclear cells from control hamsters incubated with lipopolysaccharide, 10 micrograms/ml, produced 172.5 ng TNF and 44.6 pg of IL-1/10(6) mononuclear cells/24 hr. The effect of visceral leishmaniasis on food intake was assessed in a separate group of animals housed individually in metabolic cages. Significant reductions in weight and food intake were first observed at 2 and 3 weeks of infection, respectively. By 5 weeks, the food intake of infected animals was 46% that of controls. Syrian hamsters infected with L. donovani provide an excellent model with which to study the mechanism of wasting.

Duke Scholars

Published In

Am J Trop Med Hyg

DOI

ISSN

0002-9637

Publication Date

December 1990

Volume

43

Issue

6

Start / End Page

640 / 649

Location

United States

Related Subject Headings

  • Tumor Necrosis Factor-alpha
  • Tropical Medicine
  • Spleen
  • Phagocytes
  • Mesocricetus
  • Leishmaniasis, Visceral
  • Leishmania donovani
  • Interleukin-1
  • Eating
  • Disease Models, Animal
 

Citation

APA
Chicago
ICMJE
MLA
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Pearson, R. D., Cox, G., Evans, T., Smith, D. L., Weidel, D., & Castracane, J. (1990). Wasting and macrophage production of tumor necrosis factor/cachectin and interleukin 1 in experimental visceral leishmaniasis. Am J Trop Med Hyg, 43(6), 640–649. https://doi.org/10.4269/ajtmh.1990.43.640
Pearson, R. D., G. Cox, T. Evans, D. L. Smith, D. Weidel, and J. Castracane. “Wasting and macrophage production of tumor necrosis factor/cachectin and interleukin 1 in experimental visceral leishmaniasis.Am J Trop Med Hyg 43, no. 6 (December 1990): 640–49. https://doi.org/10.4269/ajtmh.1990.43.640.
Pearson RD, Cox G, Evans T, Smith DL, Weidel D, Castracane J. Wasting and macrophage production of tumor necrosis factor/cachectin and interleukin 1 in experimental visceral leishmaniasis. Am J Trop Med Hyg. 1990 Dec;43(6):640–9.
Pearson, R. D., et al. “Wasting and macrophage production of tumor necrosis factor/cachectin and interleukin 1 in experimental visceral leishmaniasis.Am J Trop Med Hyg, vol. 43, no. 6, Dec. 1990, pp. 640–49. Pubmed, doi:10.4269/ajtmh.1990.43.640.
Pearson RD, Cox G, Evans T, Smith DL, Weidel D, Castracane J. Wasting and macrophage production of tumor necrosis factor/cachectin and interleukin 1 in experimental visceral leishmaniasis. Am J Trop Med Hyg. 1990 Dec;43(6):640–649.

Published In

Am J Trop Med Hyg

DOI

ISSN

0002-9637

Publication Date

December 1990

Volume

43

Issue

6

Start / End Page

640 / 649

Location

United States

Related Subject Headings

  • Tumor Necrosis Factor-alpha
  • Tropical Medicine
  • Spleen
  • Phagocytes
  • Mesocricetus
  • Leishmaniasis, Visceral
  • Leishmania donovani
  • Interleukin-1
  • Eating
  • Disease Models, Animal