Collagen-chondroitin sulfate-hydroxyapatite porous composites: preparation, characterization and in vitro biocompatibility testing

The aim of this study was to develop two new variants of porous composites based on collagen-chondroitin sulfate-hydroxyapatite (COL-CS-HA) mixtures. Electron microscopy examination showed that the composites had a microporous structure, with HA deposits nonhomogeneously disposed on COL fibrous bundles. Their porosity was higher than 90 % and the biodegradation in the presence of collagenase was reduced after UV treatment of the scaffolds. Composite biocompatibility was evaluated by MTT assay and light microscopy after cultivation in a human osteoblast primary culture. All COL-based materials showed a good in vitro biocompatibility, but COL-CS-HA composites presented better cell proliferation and adhesion than COL material. These porous composites demonstrate potential for future application as cell scaffolds in bone tissue engineering. Copyright © 2008 Bucharest University.