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Comparison of colloidal gold electrode fabrication methods: the preparation of a horseradish peroxidase enzyme electrode.

Publication ,  Journal Article
Stonehuerner, JG; Zhoa, J; O'Daly, JP; Crumbliss, AL; Henkens, RW
Published in: Biosensors & bioelectronics
January 1992

In order to prepare biosensing electrodes which respond to hydrogen peroxide, horseradish peroxidase has been adsorbed to colloidal gold sols and electrodes prepared by deposition of these enzyme-gold sols onto glassy carbon using three methods: evaporation, electrodeposition and electrolyte deposition. In the latter method the enzyme-gold sol is applied to the surface of a glassy carbon disk electrode followed by an equal volume of 2 mM CaCl2. The electrolyte causes the sol to precipitate on the electrode surface, producing an immobilized enzyme electrode. Satisfactory electrodes which gave an electrochemical response to hydrogen peroxide in the presence of the electron transfer mediator ferrocenecarboxylic acid were produced by all three methods. Evaporation of horseradish peroxidase-gold sols produced electrodes with the best reproducibility and the widest linear amperometric response range. These electrodes can also easily be stored in a dry state. Although not as good as evaporation, electrodeposition also produced satisfactory electrodes. Electro-deposition provides the added advantage that it lends itself to the preparation of multi-enzyme/multi-analyte electrodes by the adsorption of different enzymes to separate gold sols, followed by sequential electrodeposition onto discrete areas of a multichannel electrode.

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Published In

Biosensors & bioelectronics

DOI

EISSN

1873-4235

ISSN

0956-5663

Publication Date

January 1992

Volume

7

Issue

6

Start / End Page

421 / 428

Related Subject Headings

  • Hydrogen Peroxide
  • Horseradish Peroxidase
  • Gold
  • Evaluation Studies as Topic
  • Electrochemistry
  • Biosensing Techniques
  • Bioinformatics
  • 4018 Nanotechnology
  • 4003 Biomedical engineering
  • 3401 Analytical chemistry
 

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Stonehuerner, J. G., Zhoa, J., O’Daly, J. P., Crumbliss, A. L., & Henkens, R. W. (1992). Comparison of colloidal gold electrode fabrication methods: the preparation of a horseradish peroxidase enzyme electrode. Biosensors & Bioelectronics, 7(6), 421–428. https://doi.org/10.1016/0956-5663(92)85041-8
Stonehuerner, J. G., J. Zhoa, J. P. O’Daly, A. L. Crumbliss, and R. W. Henkens. “Comparison of colloidal gold electrode fabrication methods: the preparation of a horseradish peroxidase enzyme electrode.Biosensors & Bioelectronics 7, no. 6 (January 1992): 421–28. https://doi.org/10.1016/0956-5663(92)85041-8.
Stonehuerner JG, Zhoa J, O’Daly JP, Crumbliss AL, Henkens RW. Comparison of colloidal gold electrode fabrication methods: the preparation of a horseradish peroxidase enzyme electrode. Biosensors & bioelectronics. 1992 Jan;7(6):421–8.
Stonehuerner, J. G., et al. “Comparison of colloidal gold electrode fabrication methods: the preparation of a horseradish peroxidase enzyme electrode.Biosensors & Bioelectronics, vol. 7, no. 6, Jan. 1992, pp. 421–28. Epmc, doi:10.1016/0956-5663(92)85041-8.
Stonehuerner JG, Zhoa J, O’Daly JP, Crumbliss AL, Henkens RW. Comparison of colloidal gold electrode fabrication methods: the preparation of a horseradish peroxidase enzyme electrode. Biosensors & bioelectronics. 1992 Jan;7(6):421–428.
Journal cover image

Published In

Biosensors & bioelectronics

DOI

EISSN

1873-4235

ISSN

0956-5663

Publication Date

January 1992

Volume

7

Issue

6

Start / End Page

421 / 428

Related Subject Headings

  • Hydrogen Peroxide
  • Horseradish Peroxidase
  • Gold
  • Evaluation Studies as Topic
  • Electrochemistry
  • Biosensing Techniques
  • Bioinformatics
  • 4018 Nanotechnology
  • 4003 Biomedical engineering
  • 3401 Analytical chemistry