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Tissue-specific genetic control of splicing: Implications for the study of complex traits

Publication ,  Journal Article
Heinzen, EL; Ge, D; Cronin, KD; Maia, JM; Shianna, KV; Gabriel, WN; Welsh-Bohmer, KA; Hulette, CM; Denny, TN; Goldstein, DB
Published in: PLoS Biology
2008

Numerous genome-wide screens for polymorphisms that influence gene expression have provided key insights into the genetic control of transcription. Despite this work, the relevance of specific polymorphisms to in vivo expression and splicing remains unclear. We carried out the first genome-wide screen, to our knowledge, for SNPs that associate with alternative splicing and gene expression in human primary cells, evaluating 93 autopsy-collected cortical brain tissue samples with no defined neuropsychiatric condition and 80 peripheral blood mononucleated cell samples collected from living healthy donors. We identified 23 high confidence associations with total expression and 80 with alternative splicing as reflected by expression levels of specific exons. Fewer than 50% of the implicated SNPs however show effects in both tissue types, reflecting strong evidence for distinct genetic control of splicing and expression in the two tissue types. The data generated here also suggest the possibility that splicing effects may be responsible for up to 13 out of 84 reported genome-wide significant associations with human traits. These results emphasize the importance of establishing a database of polymorphisms affecting splicing and expression in primary tissue types and suggest that splicing effects may be of more phenotypic significance than overall gene expression changes. © 2008 Heinzen et al.

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Published In

PLoS Biology

DOI

ISSN

1544-9173

Publication Date

2008

Volume

6

Issue

12

Start / End Page

2869 / 2879

Related Subject Headings

  • Developmental Biology
  • 11 Medical and Health Sciences
  • 07 Agricultural and Veterinary Sciences
  • 06 Biological Sciences
 

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Heinzen, E. L., Ge, D., Cronin, K. D., Maia, J. M., Shianna, K. V., Gabriel, W. N., … Goldstein, D. B. (2008). Tissue-specific genetic control of splicing: Implications for the study of complex traits. PLoS Biology, 6(12), 2869–2879. https://doi.org/10.1371/journal.pbio.1000001
Heinzen, E. L., D. Ge, K. D. Cronin, J. M. Maia, K. V. Shianna, W. N. Gabriel, K. A. Welsh-Bohmer, C. M. Hulette, T. N. Denny, and D. B. Goldstein. “Tissue-specific genetic control of splicing: Implications for the study of complex traits.” PLoS Biology 6, no. 12 (2008): 2869–79. https://doi.org/10.1371/journal.pbio.1000001.
Heinzen EL, Ge D, Cronin KD, Maia JM, Shianna KV, Gabriel WN, et al. Tissue-specific genetic control of splicing: Implications for the study of complex traits. PLoS Biology. 2008;6(12):2869–79.
Heinzen, E. L., et al. “Tissue-specific genetic control of splicing: Implications for the study of complex traits.” PLoS Biology, vol. 6, no. 12, 2008, pp. 2869–79. Scival, doi:10.1371/journal.pbio.1000001.
Heinzen EL, Ge D, Cronin KD, Maia JM, Shianna KV, Gabriel WN, Welsh-Bohmer KA, Hulette CM, Denny TN, Goldstein DB. Tissue-specific genetic control of splicing: Implications for the study of complex traits. PLoS Biology. 2008;6(12):2869–2879.
Journal cover image

Published In

PLoS Biology

DOI

ISSN

1544-9173

Publication Date

2008

Volume

6

Issue

12

Start / End Page

2869 / 2879

Related Subject Headings

  • Developmental Biology
  • 11 Medical and Health Sciences
  • 07 Agricultural and Veterinary Sciences
  • 06 Biological Sciences