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Enzyme immunoassay for detection of human immunodeficiency virus-specific immunoglobulin A antibodies.

Publication ,  Journal Article
Connor, E; Wang, Z; Stephens, R; Holland, B; Palumbo, P; McSherry, G; Oleske, J; Denny, T
Published in: J Clin Microbiol
March 1993

Early diagnosis of human immunodeficiency virus (HIV) infection may be difficult in adults with acute or recent HIV infection and in infants with perinatally acquired HIV. Detection of HIV-specific immunoglobulin A (IgA) antibodies in infant serum by Western blot (immunoblot) has been suggested as a reliable method to identify HIV-infected infants, especially those over the age of 6 months, and as an adjunct to diagnosis of acute HIV infection in adults. We developed a simple enzyme immunoassay for detection of HIV-specific IgA, using standard commercially available reagents. Enzyme immunoassay was comparable to Western blot for detection of HIV-specific IgA in sera from adults (n = 216), older children (n = 49), and infants born to HIV-infected mothers (n = 65). Specificity was 100% and sensitivity ranged from 80 to 92%. IgA-enzyme immunoassay is a simple, highly sensitive method for detection of HIV-specific IgA antibodies and is easily adapted to the standard clinical laboratory.

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Published In

J Clin Microbiol

DOI

ISSN

0095-1137

Publication Date

March 1993

Volume

31

Issue

3

Start / End Page

681 / 684

Location

United States

Related Subject Headings

  • Sensitivity and Specificity
  • Predictive Value of Tests
  • Middle Aged
  • Microbiology
  • Male
  • Infant
  • Immunoglobulin A
  • Immunoenzyme Techniques
  • Humans
  • HIV-1
 

Citation

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Connor, E., Wang, Z., Stephens, R., Holland, B., Palumbo, P., McSherry, G., … Denny, T. (1993). Enzyme immunoassay for detection of human immunodeficiency virus-specific immunoglobulin A antibodies. J Clin Microbiol, 31(3), 681–684. https://doi.org/10.1128/jcm.31.3.681-684.1993
Connor, E., Z. Wang, R. Stephens, B. Holland, P. Palumbo, G. McSherry, J. Oleske, and T. Denny. “Enzyme immunoassay for detection of human immunodeficiency virus-specific immunoglobulin A antibodies.J Clin Microbiol 31, no. 3 (March 1993): 681–84. https://doi.org/10.1128/jcm.31.3.681-684.1993.
Connor E, Wang Z, Stephens R, Holland B, Palumbo P, McSherry G, et al. Enzyme immunoassay for detection of human immunodeficiency virus-specific immunoglobulin A antibodies. J Clin Microbiol. 1993 Mar;31(3):681–4.
Connor, E., et al. “Enzyme immunoassay for detection of human immunodeficiency virus-specific immunoglobulin A antibodies.J Clin Microbiol, vol. 31, no. 3, Mar. 1993, pp. 681–84. Pubmed, doi:10.1128/jcm.31.3.681-684.1993.
Connor E, Wang Z, Stephens R, Holland B, Palumbo P, McSherry G, Oleske J, Denny T. Enzyme immunoassay for detection of human immunodeficiency virus-specific immunoglobulin A antibodies. J Clin Microbiol. 1993 Mar;31(3):681–684.

Published In

J Clin Microbiol

DOI

ISSN

0095-1137

Publication Date

March 1993

Volume

31

Issue

3

Start / End Page

681 / 684

Location

United States

Related Subject Headings

  • Sensitivity and Specificity
  • Predictive Value of Tests
  • Middle Aged
  • Microbiology
  • Male
  • Infant
  • Immunoglobulin A
  • Immunoenzyme Techniques
  • Humans
  • HIV-1