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Analysis of T-cell differentiation antigens in acute lymphatic leukemia using monoclonal antibodies.

Publication ,  Journal Article
Koziner, B; Gebhard, D; Denny, T; McKenzie, S; Clarkson, BD; Miller, DA; Evans, RL
Published in: Blood
September 1982

Leukemic cells in 134 patients with ALL were analyzed by a panel of mouse monoclonal antibodies. Two antibodies are reactive with all peripheral blood T cells but define different surface antigens (Leu-1 and Leu-4). Two other antibodies react with antigens that are restricted to suppressor/cytotoxic T cells (Leu-2) and to helper T cells (Leu-3). We also used antibodies to the receptor for sheep red blood cells (SRBC) (Leu-5) and to a human "TL-like" antigen that is found on most thymocytes but not in peripheral T cells (Leu-6). An antibody to the human p29.34 "Ia-like" molecule was also tested. Of the 134 ALL patients, 17 had a predominance of SRBC-rosetting (Leu-5+) lymphoblasts ("T" ALL), expressing different surface phenotypes defined by this panel of monoclonal antibodies. These phenotypes were not readily classifiable according to a scheme of sequential stages of normal differentiation proposed. Moreover, the lymphoblasts in 8 of 113 patients not expressing conventional B- to T-cell markers ("null" ALL) reacted with the monoclonal anti-T-cell antibodies. This study suggests that the classification of lymphoblasts in ALL based on the reactivities observed with this panel of mouse monoclonal antibodies is not easily reconciled with current models of normal T-cell differentiation. However, it should be emphasized that the precise sequence of antigenic expression by cells undergoing thymic differentiation is still not fully known, and further phenotypic analysis of ALL cells might contribute to an improved understanding of this malignancy.

Duke Scholars

Published In

Blood

ISSN

0006-4971

Publication Date

September 1982

Volume

60

Issue

3

Start / End Page

752 / 757

Location

United States

Related Subject Headings

  • T-Lymphocytes
  • Rosette Formation
  • Receptors, Antigen, B-Cell
  • Phenotype
  • Mice
  • Male
  • Lymphocytes, Null
  • Leukemia, Lymphoid
  • Infant
  • Immunology
 

Citation

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Koziner, B., Gebhard, D., Denny, T., McKenzie, S., Clarkson, B. D., Miller, D. A., & Evans, R. L. (1982). Analysis of T-cell differentiation antigens in acute lymphatic leukemia using monoclonal antibodies. Blood, 60(3), 752–757.
Koziner, B., D. Gebhard, T. Denny, S. McKenzie, B. D. Clarkson, D. A. Miller, and R. L. Evans. “Analysis of T-cell differentiation antigens in acute lymphatic leukemia using monoclonal antibodies.Blood 60, no. 3 (September 1982): 752–57.
Koziner B, Gebhard D, Denny T, McKenzie S, Clarkson BD, Miller DA, et al. Analysis of T-cell differentiation antigens in acute lymphatic leukemia using monoclonal antibodies. Blood. 1982 Sep;60(3):752–7.
Koziner, B., et al. “Analysis of T-cell differentiation antigens in acute lymphatic leukemia using monoclonal antibodies.Blood, vol. 60, no. 3, Sept. 1982, pp. 752–57.
Koziner B, Gebhard D, Denny T, McKenzie S, Clarkson BD, Miller DA, Evans RL. Analysis of T-cell differentiation antigens in acute lymphatic leukemia using monoclonal antibodies. Blood. 1982 Sep;60(3):752–757.

Published In

Blood

ISSN

0006-4971

Publication Date

September 1982

Volume

60

Issue

3

Start / End Page

752 / 757

Location

United States

Related Subject Headings

  • T-Lymphocytes
  • Rosette Formation
  • Receptors, Antigen, B-Cell
  • Phenotype
  • Mice
  • Male
  • Lymphocytes, Null
  • Leukemia, Lymphoid
  • Infant
  • Immunology