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Comparative time-course of benzo[a]pyrene-DNA adduct formation, and its relationship to CYP1A activity in two species of catfish

Publication ,  Journal Article
Ploch, SA; King, LC; Di Giulio, RT
Published in: Marine Environmental Research
July 1, 1998

Studies have demonstrated differences between the brown bullhead (Ameriurus nebulosus) (BB) and a closely related species of catfish, the channel catfish (Ictalurus punctatus) (CC) in activity of important liver enzymes that may affect their ability to activate or detoxify certain procarcinogens. We have measured the formation and persistence of benzo[a]pyrene-DNA adducts in the liver of these two species using the 32P-postlabeling (PPL) method, along with the effect of prior induction by β-naphthoflavone (βNF). In this time-course study, a total of 80 fish from each species were used. Half were induced with βNF (i.p. injections of 10 mg kg-1 at day 3 and 2) followed by a single benzo[a]pyrene (BaP) i.p. injection (20 mg kg-1). Liver DNA and microsomes were sampled on day 1, 3, 7, 14 and 45 after the BaP dosage - adducts and ethoxyresorufin-O-deethylase (EROD) activity were analyzed. EROD activity in CC was significantly higher at most time points than in BB. In contrast, the BaP-DNA adduct profile revealed a significantly higher level of adducts in the BB than the CC. Adduct levels at most time points were significantly higher in the BB than in the CC at p < 0.05. Further characterization of the major adduct by HPLC confirmed it to be the anti-BPDE-dG adduct. The adduct profiles of BaP are in contrast to what has been observed for adducts of 2-aminoanthracene in these species, although it is consistent with the relative species susceptibility to PAH induced liver neoplasia.

Duke Scholars

Published In

Marine Environmental Research

DOI

ISSN

0141-1136

Publication Date

July 1, 1998

Volume

46

Issue

1-5

Start / End Page

345 / 349

Related Subject Headings

  • Marine Biology & Hydrobiology
  • 41 Environmental sciences
  • 34 Chemical sciences
  • 31 Biological sciences
  • 06 Biological Sciences
  • 05 Environmental Sciences
  • 03 Chemical Sciences
 

Citation

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ICMJE
MLA
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Ploch, S. A., King, L. C., & Di Giulio, R. T. (1998). Comparative time-course of benzo[a]pyrene-DNA adduct formation, and its relationship to CYP1A activity in two species of catfish. Marine Environmental Research, 46(1–5), 345–349. https://doi.org/10.1016/S0141-1136(97)00118-9
Ploch, S. A., L. C. King, and R. T. Di Giulio. “Comparative time-course of benzo[a]pyrene-DNA adduct formation, and its relationship to CYP1A activity in two species of catfish.” Marine Environmental Research 46, no. 1–5 (July 1, 1998): 345–49. https://doi.org/10.1016/S0141-1136(97)00118-9.
Ploch SA, King LC, Di Giulio RT. Comparative time-course of benzo[a]pyrene-DNA adduct formation, and its relationship to CYP1A activity in two species of catfish. Marine Environmental Research. 1998 Jul 1;46(1–5):345–9.
Ploch, S. A., et al. “Comparative time-course of benzo[a]pyrene-DNA adduct formation, and its relationship to CYP1A activity in two species of catfish.” Marine Environmental Research, vol. 46, no. 1–5, July 1998, pp. 345–49. Scopus, doi:10.1016/S0141-1136(97)00118-9.
Ploch SA, King LC, Di Giulio RT. Comparative time-course of benzo[a]pyrene-DNA adduct formation, and its relationship to CYP1A activity in two species of catfish. Marine Environmental Research. 1998 Jul 1;46(1–5):345–349.
Journal cover image

Published In

Marine Environmental Research

DOI

ISSN

0141-1136

Publication Date

July 1, 1998

Volume

46

Issue

1-5

Start / End Page

345 / 349

Related Subject Headings

  • Marine Biology & Hydrobiology
  • 41 Environmental sciences
  • 34 Chemical sciences
  • 31 Biological sciences
  • 06 Biological Sciences
  • 05 Environmental Sciences
  • 03 Chemical Sciences