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Phenotypic abnormalities in macrophages from leptin-deficient, obese mice.

Publication ,  Journal Article
Lee, FY; Li, Y; Yang, EK; Yang, SQ; Lin, HZ; Trush, MA; Dannenberg, AJ; Diehl, AM
Published in: Am J Physiol
February 1999

Obesity is a complex syndrome that involves defective signaling by a number of different factors that regulate appetite and energy homeostasis. Treatment with exogenous leptin reverses hyperphagia and obesity in ob/ob mice, which have a mutation that causes leptin deficiency, proving the importance of this factor and its receptors in the obesity syndrome. Cells with leptin receptors have been identified outside of the appetite regulatory centers in the brain. Thus leptin has peripheral targets. Because macrophages express signaling-competent leptin receptors, these cells may be altered during chronic leptin deficiency. Consistent with this concept, the present study identifies several phenotypic abnormalities in macrophages from ob/ob mice, including decreased steady-state levels of uncoupling protein-2 mRNA, increased mitochondrial production of superoxide and hydrogen peroxide, constitutive activation of CCAAT enhancer binding protein (C/EBP)-beta, an oxidant-sensitive transcription factor, increased expression of interleukin-6 and cyclooxygenase (COX)-2, two C/EBP-beta target genes, and increased COX-2-dependent production of PGE2. Given the importance of macrophages in the general regulation of inflammation and immunity, these alterations in macrophage function may contribute to obesity-related pathophysiology.

Duke Scholars

Published In

Am J Physiol

DOI

ISSN

0002-9513

Publication Date

February 1999

Volume

276

Issue

2

Start / End Page

C386 / C394

Location

United States

Related Subject Headings

  • Uncoupling Protein 2
  • Superoxides
  • RNA, Messenger
  • Proteins
  • Prostaglandin-Endoperoxide Synthases
  • Phenotype
  • Obesity
  • Nuclear Proteins
  • Mitochondrial Proteins
  • Mitochondria
 

Citation

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Lee, F. Y., Li, Y., Yang, E. K., Yang, S. Q., Lin, H. Z., Trush, M. A., … Diehl, A. M. (1999). Phenotypic abnormalities in macrophages from leptin-deficient, obese mice. Am J Physiol, 276(2), C386–C394. https://doi.org/10.1152/ajpcell.1999.276.2.C386
Lee, F. Y., Y. Li, E. K. Yang, S. Q. Yang, H. Z. Lin, M. A. Trush, A. J. Dannenberg, and A. M. Diehl. “Phenotypic abnormalities in macrophages from leptin-deficient, obese mice.Am J Physiol 276, no. 2 (February 1999): C386–94. https://doi.org/10.1152/ajpcell.1999.276.2.C386.
Lee FY, Li Y, Yang EK, Yang SQ, Lin HZ, Trush MA, et al. Phenotypic abnormalities in macrophages from leptin-deficient, obese mice. Am J Physiol. 1999 Feb;276(2):C386–94.
Lee, F. Y., et al. “Phenotypic abnormalities in macrophages from leptin-deficient, obese mice.Am J Physiol, vol. 276, no. 2, Feb. 1999, pp. C386–94. Pubmed, doi:10.1152/ajpcell.1999.276.2.C386.
Lee FY, Li Y, Yang EK, Yang SQ, Lin HZ, Trush MA, Dannenberg AJ, Diehl AM. Phenotypic abnormalities in macrophages from leptin-deficient, obese mice. Am J Physiol. 1999 Feb;276(2):C386–C394.

Published In

Am J Physiol

DOI

ISSN

0002-9513

Publication Date

February 1999

Volume

276

Issue

2

Start / End Page

C386 / C394

Location

United States

Related Subject Headings

  • Uncoupling Protein 2
  • Superoxides
  • RNA, Messenger
  • Proteins
  • Prostaglandin-Endoperoxide Synthases
  • Phenotype
  • Obesity
  • Nuclear Proteins
  • Mitochondrial Proteins
  • Mitochondria