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Bacterial lipopolysaccharide induces uncoupling protein-2 expression in hepatocytes by a tumor necrosis factor-alpha-dependent mechanism.

Publication ,  Journal Article
Cortez-Pinto, H; Yang, SQ; Lin, HZ; Costa, S; Hwang, CS; Lane, MD; Bagby, G; Diehl, AM
Published in: Biochem Biophys Res Commun
October 9, 1998

The liver is a target for bacterial lipopolysaccharide (LPS) and participates in the metabolic response to endotoxemia. Recently published evidence indicates that LPS increases the expression of mitochondrial uncoupling protein-2 (UCP-2) mRNAs in several tissues, including the liver. Because hepatocytes in the healthy liver do not express UCP-2, LPS was thought to induce UCP-2 in liver macrophages, which express UCP-2 constitutively. However, the present studies of cultured peritoneal macrophages indicate that LPS reduces steady state levels of UCP-2 mRNAs in these cells. In contrast, UCP-2 mRNAs are induced in hepatocytes isolated from LPS treated rats and transfection of these hepatocytes with UCP-2 promoter-reporter constructs demonstrates substantial increases in UCP-2 promoter activity. LPS induction of hepatocyte UCP-2 expression is virtually abolished by prior treatment of rats with neutralizing antibodies to tumor necrosis factor alpha (TNF). Futhermore, TNFalpha treatment induces UCP-2 mRNA accumulation in primary cultures of hepatocytes from healthy rats. Thus, hepatocytes are likely to be important contributors to endotoxin-related increases in liver UCP-2 via a mechanism that involves the LPS-inducible cytokine, TNFalpha.

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Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

October 9, 1998

Volume

251

Issue

1

Start / End Page

313 / 319

Location

United States

Related Subject Headings

  • Uncoupling Protein 2
  • Uncoupling Agents
  • Tumor Necrosis Factor-alpha
  • Rats, Sprague-Dawley
  • Rats
  • RNA, Messenger
  • RNA
  • Protein Biosynthesis
  • Mitochondrial Proteins
  • Mice, Obese
 

Citation

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Cortez-Pinto, H., Yang, S. Q., Lin, H. Z., Costa, S., Hwang, C. S., Lane, M. D., … Diehl, A. M. (1998). Bacterial lipopolysaccharide induces uncoupling protein-2 expression in hepatocytes by a tumor necrosis factor-alpha-dependent mechanism. Biochem Biophys Res Commun, 251(1), 313–319. https://doi.org/10.1006/bbrc.1998.9473
Cortez-Pinto, H., S. Q. Yang, H. Z. Lin, S. Costa, C. S. Hwang, M. D. Lane, G. Bagby, and A. M. Diehl. “Bacterial lipopolysaccharide induces uncoupling protein-2 expression in hepatocytes by a tumor necrosis factor-alpha-dependent mechanism.Biochem Biophys Res Commun 251, no. 1 (October 9, 1998): 313–19. https://doi.org/10.1006/bbrc.1998.9473.
Cortez-Pinto H, Yang SQ, Lin HZ, Costa S, Hwang CS, Lane MD, et al. Bacterial lipopolysaccharide induces uncoupling protein-2 expression in hepatocytes by a tumor necrosis factor-alpha-dependent mechanism. Biochem Biophys Res Commun. 1998 Oct 9;251(1):313–9.
Cortez-Pinto, H., et al. “Bacterial lipopolysaccharide induces uncoupling protein-2 expression in hepatocytes by a tumor necrosis factor-alpha-dependent mechanism.Biochem Biophys Res Commun, vol. 251, no. 1, Oct. 1998, pp. 313–19. Pubmed, doi:10.1006/bbrc.1998.9473.
Cortez-Pinto H, Yang SQ, Lin HZ, Costa S, Hwang CS, Lane MD, Bagby G, Diehl AM. Bacterial lipopolysaccharide induces uncoupling protein-2 expression in hepatocytes by a tumor necrosis factor-alpha-dependent mechanism. Biochem Biophys Res Commun. 1998 Oct 9;251(1):313–319.
Journal cover image

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

October 9, 1998

Volume

251

Issue

1

Start / End Page

313 / 319

Location

United States

Related Subject Headings

  • Uncoupling Protein 2
  • Uncoupling Agents
  • Tumor Necrosis Factor-alpha
  • Rats, Sprague-Dawley
  • Rats
  • RNA, Messenger
  • RNA
  • Protein Biosynthesis
  • Mitochondrial Proteins
  • Mice, Obese