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Tumor necrosis factor alpha promotes nuclear localization of cytokine-inducible CCAAT/enhancer binding protein isoforms in hepatocytes.

Publication ,  Journal Article
Yin, M; Yang, SQ; Lin, HZ; Lane, MD; Chatterjee, S; Diehl, AM
Published in: J Biol Chem
July 26, 1996

Hepatocytes were cultured in the presence of recombinant tumor necrosis factor (TNF) alpha or mutated TNF alpha peptides that specifically activate either p55 or p75 TNF receptors to determine if TNF alpha can activate cytokine-inducible CCAAT/enhancer binding protein (C/EBP) isoforms by post-transcriptional mechanisms that are initiated by TNF receptors. Within 5-10 min after treatment with any of these agents, nuclear concentrations of C/EBP beta and C/EBP delta double and remain 2-4-fold greater than control cultures for 30 min (p < 0.01). Consistent with these results, gel mobility shift assays demonstrate 3-fold increased nuclear C/EBP beta- and C/EBP delta-DNA binding activity in TNF alpha-treated cells, and immunocytochemistry confirms rapid redistribution of these C/EBP isoforms into the nucleus. In contrast, mRNA and whole cell protein concentrations of C/EBP beta and delta are not altered by TNF alpha exposure, and nuclear concentrations of another C/EBP isoform, C/EBP alpha, are decreased by 80%. This novel evidence that TNF alpha initiates post-transcriptional activation of cytokine-inducible C/EBP isoforms identifies a mechanism that enables hepatocytes to respond immediately to inflammatory stress.

Duke Scholars

Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

July 26, 1996

Volume

271

Issue

30

Start / End Page

17974 / 17978

Location

United States

Related Subject Headings

  • Tumor Necrosis Factor-alpha
  • Receptors, Tumor Necrosis Factor
  • Protein Binding
  • Nuclear Proteins
  • Molecular Sequence Data
  • Liver
  • DNA-Binding Proteins
  • Cell Nucleus
  • Cell Compartmentation
  • CCAAT-Enhancer-Binding Proteins
 

Citation

APA
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ICMJE
MLA
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Yin, M., Yang, S. Q., Lin, H. Z., Lane, M. D., Chatterjee, S., & Diehl, A. M. (1996). Tumor necrosis factor alpha promotes nuclear localization of cytokine-inducible CCAAT/enhancer binding protein isoforms in hepatocytes. J Biol Chem, 271(30), 17974–17978. https://doi.org/10.1074/jbc.271.30.17974
Yin, M., S. Q. Yang, H. Z. Lin, M. D. Lane, S. Chatterjee, and A. M. Diehl. “Tumor necrosis factor alpha promotes nuclear localization of cytokine-inducible CCAAT/enhancer binding protein isoforms in hepatocytes.J Biol Chem 271, no. 30 (July 26, 1996): 17974–78. https://doi.org/10.1074/jbc.271.30.17974.
Yin M, Yang SQ, Lin HZ, Lane MD, Chatterjee S, Diehl AM. Tumor necrosis factor alpha promotes nuclear localization of cytokine-inducible CCAAT/enhancer binding protein isoforms in hepatocytes. J Biol Chem. 1996 Jul 26;271(30):17974–8.
Yin, M., et al. “Tumor necrosis factor alpha promotes nuclear localization of cytokine-inducible CCAAT/enhancer binding protein isoforms in hepatocytes.J Biol Chem, vol. 271, no. 30, July 1996, pp. 17974–78. Pubmed, doi:10.1074/jbc.271.30.17974.
Yin M, Yang SQ, Lin HZ, Lane MD, Chatterjee S, Diehl AM. Tumor necrosis factor alpha promotes nuclear localization of cytokine-inducible CCAAT/enhancer binding protein isoforms in hepatocytes. J Biol Chem. 1996 Jul 26;271(30):17974–17978.

Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

July 26, 1996

Volume

271

Issue

30

Start / End Page

17974 / 17978

Location

United States

Related Subject Headings

  • Tumor Necrosis Factor-alpha
  • Receptors, Tumor Necrosis Factor
  • Protein Binding
  • Nuclear Proteins
  • Molecular Sequence Data
  • Liver
  • DNA-Binding Proteins
  • Cell Nucleus
  • Cell Compartmentation
  • CCAAT-Enhancer-Binding Proteins