Skip to main content
Journal cover image

Requirement for myeloid growth factors in the differentiation of acute promyelocytic leukaemia.

Publication ,  Journal Article
Matsui, W; Smith, BD; Vala, M; Beal, N; Huff, CA; Diehl, LF; Jones, RJ
Published in: Br J Haematol
March 2005

It is well known that the differentiation of acute promyelocytic leukaemia (APL) cells by all-trans-retinoic acid (ATRA) may be enhanced by myeloid growth factors, but the requirement for growth factors in this process is unclear. Our previous studies in multiple myeloma and non-APL acute myeloid leukaemia demonstrated that lineage-specific growth factors are required for the maximal activity of many pharmacologic differentiating agents in vitro. Thus, we studied whether the differentiation of APL is similarly dependent on growth factors. We found that the myeloid growth factors granulocyte colony-stimulating factor or granulocyte-macrophage colony-stimulating factor markedly increased the differentiation of NB4 cells or APL blasts from clinical samples treated with ATRA, arsenic trioxide (ATO), or bryostatin-1 as evidenced by the enhanced expression of myeloid surface antigens and the inhibition of clonogenic growth. Furthermore, myeloid growth factors were necessary for the differentiation of APL cells since the activity of each pharmacologic agent could be blocked by specific growth factor-neutralizing antibodies. Each differentiating agent was active only at concentrations that inhibited cell cycling, suggesting that this property is also required for differentiation. These data demonstrate that both pharmacologic differentiating agents and myeloid growth factors are required, but neither sufficient, for the differentiation of APL cells. The combined use of pharmacologic differentiating agents and growth factors may improve the clinical efficacy of differentiation therapy in APL.

Duke Scholars

Published In

Br J Haematol

DOI

ISSN

0007-1048

Publication Date

March 2005

Volume

128

Issue

6

Start / End Page

853 / 862

Location

England

Related Subject Headings

  • Tretinoin
  • Oxides
  • Myelopoiesis
  • Macrolides
  • Leukemia, Promyelocytic, Acute
  • Immunology
  • Humans
  • Growth Inhibitors
  • Granulocyte-Macrophage Colony-Stimulating Factor
  • Granulocyte Colony-Stimulating Factor
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Matsui, W., Smith, B. D., Vala, M., Beal, N., Huff, C. A., Diehl, L. F., & Jones, R. J. (2005). Requirement for myeloid growth factors in the differentiation of acute promyelocytic leukaemia. Br J Haematol, 128(6), 853–862. https://doi.org/10.1111/j.1365-2141.2005.05395.x
Matsui, William, B Douglas Smith, Milada Vala, Nikeshia Beal, Carol Ann Huff, Louis F. Diehl, and Richard J. Jones. “Requirement for myeloid growth factors in the differentiation of acute promyelocytic leukaemia.Br J Haematol 128, no. 6 (March 2005): 853–62. https://doi.org/10.1111/j.1365-2141.2005.05395.x.
Matsui W, Smith BD, Vala M, Beal N, Huff CA, Diehl LF, et al. Requirement for myeloid growth factors in the differentiation of acute promyelocytic leukaemia. Br J Haematol. 2005 Mar;128(6):853–62.
Matsui, William, et al. “Requirement for myeloid growth factors in the differentiation of acute promyelocytic leukaemia.Br J Haematol, vol. 128, no. 6, Mar. 2005, pp. 853–62. Pubmed, doi:10.1111/j.1365-2141.2005.05395.x.
Matsui W, Smith BD, Vala M, Beal N, Huff CA, Diehl LF, Jones RJ. Requirement for myeloid growth factors in the differentiation of acute promyelocytic leukaemia. Br J Haematol. 2005 Mar;128(6):853–862.
Journal cover image

Published In

Br J Haematol

DOI

ISSN

0007-1048

Publication Date

March 2005

Volume

128

Issue

6

Start / End Page

853 / 862

Location

England

Related Subject Headings

  • Tretinoin
  • Oxides
  • Myelopoiesis
  • Macrolides
  • Leukemia, Promyelocytic, Acute
  • Immunology
  • Humans
  • Growth Inhibitors
  • Granulocyte-Macrophage Colony-Stimulating Factor
  • Granulocyte Colony-Stimulating Factor