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Fluid shear stress differentially modulates expression of genes encoding basic fibroblast growth factor and platelet-derived growth factor B chain in vascular endothelium.

Publication ,  Journal Article
Malek, AM; Gibbons, GH; Dzau, VJ; Izumo, S
Published in: J Clin Invest
October 1993

Fluid shear stress has been shown to be an important regulator of vascular structure and function through its effect on the endothelial cell. We have explored the effect of shear stress on the expression of the heparin-binding growth factors platelet-derived growth factor B chain (PDGF-B) and basic fibroblast growth factor (bFGF) in bovine aortic endothelial cells using a purpose-built cone-plate viscometer. Using morphometric analysis, we have mimicked the endothelial cell shape changes encountered in vivo in response to shear stress and correlated these with changes in gene expression. Steady laminar shear stress of 15 and 36 dyn/cm2 both resulted in endothelial cell shape change, but the higher shear stress induced greater and more uniform alignment in the direction of flow and nuclear protrusion after 24 h. Steady laminar shear stress of both 15 and 36 dyn/cm2 induced a significant 3.9- and 4.2-fold decrease, respectively, in PDGF-B mRNA at 9 h. In contrast, steady laminar shear of 15 dyn/cm2 induced a mild and transient 1.5-fold increase in bFGF mRNA while shear of 36 dyn/cm2 induced a significant 4.8-fold increase at 6 h of shear which remained at 2.9-fold at 9 h. Pulsatile and turbulent shear stress showed the same effect as steady laminar shear stress (all at 15 dyn/cm2 time-average magnitude) on PDGF-B and bFGF mRNA content. Cyclic stretch (20% strain, 20/min) of cells grown on silicone substrate did not significantly affect either PDGF-B or bFGF mRNA levels. These results suggest that expression of each peptide growth factor gene is differentially regulated by fluid shear stress in the vascular endothelial cell. These results may have implications on vascular structure and function in response to hemodynamic forces and present a model for the study of transduction of mechanical stimuli into altered gene expression.

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Published In

J Clin Invest

DOI

ISSN

0021-9738

Publication Date

October 1993

Volume

92

Issue

4

Start / End Page

2013 / 2021

Location

United States

Related Subject Headings

  • Stress, Mechanical
  • RNA, Messenger
  • Platelet-Derived Growth Factor
  • Macromolecular Substances
  • Immunology
  • Gene Expression Regulation
  • Fibroblast Growth Factor 2
  • Endothelium, Vascular
  • DNA Probes
  • Cells, Cultured
 

Citation

APA
Chicago
ICMJE
MLA
NLM

Published In

J Clin Invest

DOI

ISSN

0021-9738

Publication Date

October 1993

Volume

92

Issue

4

Start / End Page

2013 / 2021

Location

United States

Related Subject Headings

  • Stress, Mechanical
  • RNA, Messenger
  • Platelet-Derived Growth Factor
  • Macromolecular Substances
  • Immunology
  • Gene Expression Regulation
  • Fibroblast Growth Factor 2
  • Endothelium, Vascular
  • DNA Probes
  • Cells, Cultured