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Characterization of purified rabbit uterine renin: influence of pregnancy on uterine inactive renin.

Publication ,  Journal Article
Dzau, VJ; Gonzalez, D; Ellison, K; Churchill, S; Emmett, N
Published in: Endocrinology
January 1987

Using specific antibody raised against renal renin, we have documented that the majority of the uterine renin-like activity in gravid and nongravid uteri is immunoreactive renin. To characterize its physiochemical properties, we obtained highly purified uterine renin by two affinity chromatographic steps, pepstatin and antirenin. Uterine renin has a pH optimum of 6, an apparent mol wt of 38K, and a Km of 1.7 microM for homologous substrate. These properties are identical to those of renal renin and are not influenced by the pregnant state. In the basal state, an inactive form of the uterine enzyme constitute 55 +/- 10% of the total uterine renin. During pregnancy, active renin increased 40-fold as inactive renin fell to 4 +/- 3% of the total renin concentration. The renal renin concentration fell as plasma renin increased during pregnancy. These data suggest that the increased uterine renin concentrations during pregnancy are probably due to increased local production and conversion of renin precursor to the active enzyme. This stimulation of the uterine renin level appears to be independent of renal renin.

Duke Scholars

Published In

Endocrinology

DOI

ISSN

0013-7227

Publication Date

January 1987

Volume

120

Issue

1

Start / End Page

358 / 364

Location

United States

Related Subject Headings

  • Uterus
  • Renin
  • Rabbits
  • Pregnancy, Animal
  • Pregnancy
  • Molecular Weight
  • Kinetics
  • Kidney
  • Hydrogen-Ion Concentration
  • Female
 

Citation

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Dzau, V. J., Gonzalez, D., Ellison, K., Churchill, S., & Emmett, N. (1987). Characterization of purified rabbit uterine renin: influence of pregnancy on uterine inactive renin. Endocrinology, 120(1), 358–364. https://doi.org/10.1210/endo-120-1-358
Dzau, V. J., D. Gonzalez, K. Ellison, S. Churchill, and N. Emmett. “Characterization of purified rabbit uterine renin: influence of pregnancy on uterine inactive renin.Endocrinology 120, no. 1 (January 1987): 358–64. https://doi.org/10.1210/endo-120-1-358.
Dzau VJ, Gonzalez D, Ellison K, Churchill S, Emmett N. Characterization of purified rabbit uterine renin: influence of pregnancy on uterine inactive renin. Endocrinology. 1987 Jan;120(1):358–64.
Dzau, V. J., et al. “Characterization of purified rabbit uterine renin: influence of pregnancy on uterine inactive renin.Endocrinology, vol. 120, no. 1, Jan. 1987, pp. 358–64. Pubmed, doi:10.1210/endo-120-1-358.
Dzau VJ, Gonzalez D, Ellison K, Churchill S, Emmett N. Characterization of purified rabbit uterine renin: influence of pregnancy on uterine inactive renin. Endocrinology. 1987 Jan;120(1):358–364.
Journal cover image

Published In

Endocrinology

DOI

ISSN

0013-7227

Publication Date

January 1987

Volume

120

Issue

1

Start / End Page

358 / 364

Location

United States

Related Subject Headings

  • Uterus
  • Renin
  • Rabbits
  • Pregnancy, Animal
  • Pregnancy
  • Molecular Weight
  • Kinetics
  • Kidney
  • Hydrogen-Ion Concentration
  • Female