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Healing of cultured human cornea after free electron laser ablation

Publication ,  Journal Article
Robinson, RD; Shen, JH; Joos, KM; Shetlar, DJ; Edwards, GS; O'Day, DM
Published in: Investigative Ophthalmology and Visual Science
1997

Purpose. To study the healing process in cultured human corneas after Qswitched Er:YAG laser ablation. Methods. Fresh human cadaver corneas within were ablated with a Q-switched Er:YAG laser at 2.94 m wavelength. (100ns, 1 Hz) The radiant exposure was 500 mJ/cm2. A focal crater was created using between 60 and 80 pulses. A linear incision was also formed by slowly scanning the laser over the corneal surface. Each cornea was cultured on a tissue supporting frame immediately after the ablation. Culture media consisted of 92% minimum essential media, 8% fetal bovine serum, 0.125% HEPES buffer solution, 0.125% gentamicin, and 0.05% fungizone. The entire tissue frame and media container were kept in an incubator at 37°C and 5% C02. Serial macroscopic photographs of the cultured corneas were taken during the healing process. Histology was performed after 17 days of culture. Results. At 3 days, a transient haze was observed within the craters which then resolved between 7 and 14 days. No haze was seen along the linear incision. Histologie sections of the cornea showed complete re-epithelialization of the lased area. The endothelium appeared unaffected. Conclusions. Cultured human corneas may provide useful information regarding the healing process following laser ablation.

Duke Scholars

Published In

Investigative Ophthalmology and Visual Science

ISSN

0146-0404

Publication Date

1997

Volume

38

Issue

4

Start / End Page

S406

Related Subject Headings

  • Ophthalmology & Optometry
  • 11 Medical and Health Sciences
  • 06 Biological Sciences
 

Citation

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Robinson, R. D., Shen, J. H., Joos, K. M., Shetlar, D. J., Edwards, G. S., & O’Day, D. M. (1997). Healing of cultured human cornea after free electron laser ablation. Investigative Ophthalmology and Visual Science, 38(4), S406.
Robinson, R. D., J. H. Shen, K. M. Joos, D. J. Shetlar, G. S. Edwards, and D. M. O’Day. “Healing of cultured human cornea after free electron laser ablation.” Investigative Ophthalmology and Visual Science 38, no. 4 (1997): S406.
Robinson RD, Shen JH, Joos KM, Shetlar DJ, Edwards GS, O’Day DM. Healing of cultured human cornea after free electron laser ablation. Investigative Ophthalmology and Visual Science. 1997;38(4):S406.
Robinson, R. D., et al. “Healing of cultured human cornea after free electron laser ablation.” Investigative Ophthalmology and Visual Science, vol. 38, no. 4, 1997, p. S406.
Robinson RD, Shen JH, Joos KM, Shetlar DJ, Edwards GS, O’Day DM. Healing of cultured human cornea after free electron laser ablation. Investigative Ophthalmology and Visual Science. 1997;38(4):S406.

Published In

Investigative Ophthalmology and Visual Science

ISSN

0146-0404

Publication Date

1997

Volume

38

Issue

4

Start / End Page

S406

Related Subject Headings

  • Ophthalmology & Optometry
  • 11 Medical and Health Sciences
  • 06 Biological Sciences