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Role of immersion refractometry for investigating laser-induced effects in cells.

Publication ,  Journal Article
Tribble, J; Kozub, J; Aly, A; Ossoff, R; Edwards, G
Published in: Lasers in surgery and medicine
January 1992

The broad background of scattered light observed in spectra of cell suspensions is reduced by factors of up to 20 by immersion refractometry allowing for improved spectroscopic determination of the absorption properties of cells in the 325-820 nm range. Refractive-index matched spectra of E. coli C1a exhibit a set of resonant features near 422, 561, and 582 nm. Exposure wavelengths are chosen based on this spectrum and cell viability is investigated in E. coli suspensions exposed to 350, 400, 422, 440, and 700 nm radiation delivered in nanosecond pulses with total doses from 500 millijoules to 60 Joules. We observe a loss in cell viability for doses greater than 1 Joule at 422 nm and for all doses at other wavelengths; exposures of less than 1 Joule at 422 nm enhance growth. Excluding exposures at wavelengths within the resonant feature, longer wavelengths are less effective at reducing the viability of E. coli C1a. This indicates the occurrence of at least two absorption processes.

Duke Scholars

Published In

Lasers in surgery and medicine

DOI

EISSN

1096-9101

ISSN

0196-8092

Publication Date

January 1992

Volume

12

Issue

4

Start / End Page

459 / 463

Related Subject Headings

  • Yttrium
  • Spectrum Analysis
  • Scattering, Radiation
  • Refractometry
  • Radiation Dosage
  • Neodymium
  • Laser Therapy
  • Immersion
  • Escherichia coli
  • Dermatology & Venereal Diseases
 

Citation

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Tribble, J., Kozub, J., Aly, A., Ossoff, R., & Edwards, G. (1992). Role of immersion refractometry for investigating laser-induced effects in cells. Lasers in Surgery and Medicine, 12(4), 459–463. https://doi.org/10.1002/lsm.1900120416
Tribble, J., J. Kozub, A. Aly, R. Ossoff, and G. Edwards. “Role of immersion refractometry for investigating laser-induced effects in cells.Lasers in Surgery and Medicine 12, no. 4 (January 1992): 459–63. https://doi.org/10.1002/lsm.1900120416.
Tribble J, Kozub J, Aly A, Ossoff R, Edwards G. Role of immersion refractometry for investigating laser-induced effects in cells. Lasers in surgery and medicine. 1992 Jan;12(4):459–63.
Tribble, J., et al. “Role of immersion refractometry for investigating laser-induced effects in cells.Lasers in Surgery and Medicine, vol. 12, no. 4, Jan. 1992, pp. 459–63. Epmc, doi:10.1002/lsm.1900120416.
Tribble J, Kozub J, Aly A, Ossoff R, Edwards G. Role of immersion refractometry for investigating laser-induced effects in cells. Lasers in surgery and medicine. 1992 Jan;12(4):459–463.
Journal cover image

Published In

Lasers in surgery and medicine

DOI

EISSN

1096-9101

ISSN

0196-8092

Publication Date

January 1992

Volume

12

Issue

4

Start / End Page

459 / 463

Related Subject Headings

  • Yttrium
  • Spectrum Analysis
  • Scattering, Radiation
  • Refractometry
  • Radiation Dosage
  • Neodymium
  • Laser Therapy
  • Immersion
  • Escherichia coli
  • Dermatology & Venereal Diseases