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Spindle dynamics during meiosis in Drosophila oocytes.

Publication ,  Journal Article
Endow, SA; Komma, DJ
Published in: J Cell Biol
June 16, 1997

Mature oocytes of Drosophila are arrested in metaphase of meiosis I. Upon activation by ovulation or fertilization, oocytes undergo a series of rapid changes that have not been directly visualized previously. We report here the use of the Nonclaret disjunctional (Ncd) microtubule motor protein fused to the green fluorescent protein (GFP) to monitor changes in the meiotic spindle of live oocytes after activation in vitro. Meiotic spindles of metaphase-arrested oocytes are relatively stable, however, meiotic spindles of in vitro-activated oocytes are highly dynamic: the spindles elongate, rotate around their long axis, and undergo an acute pivoting movement to reorient perpendicular to the oocyte surface. Many oocytes spontaneously complete the meiotic divisions, permitting visualization of progression from meiosis I to II. The movements of the spindle after oocyte activation provide new information about the dynamic changes in the spindle that occur upon re-entry into meiosis and completion of the meiotic divisions. Spindles in live oocytes mutant for a loss-of-function ncd allele fused to gfp were also imaged. The genesis of spindle defects in the live mutant oocytes provides new insights into the mechanism of Ncd function in the spindle during the meiotic divisions.

Duke Scholars

Published In

J Cell Biol

DOI

ISSN

0021-9525

Publication Date

June 16, 1997

Volume

137

Issue

6

Start / End Page

1321 / 1336

Location

United States

Related Subject Headings

  • Transgenes
  • Spindle Apparatus
  • Recombinant Fusion Proteins
  • Oocytes
  • Mutation
  • Microtubule Proteins
  • Meiosis
  • Luminescent Proteins
  • Kinesins
  • Green Fluorescent Proteins
 

Citation

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Endow, S. A., & Komma, D. J. (1997). Spindle dynamics during meiosis in Drosophila oocytes. J Cell Biol, 137(6), 1321–1336. https://doi.org/10.1083/jcb.137.6.1321
Endow, S. A., and D. J. Komma. “Spindle dynamics during meiosis in Drosophila oocytes.J Cell Biol 137, no. 6 (June 16, 1997): 1321–36. https://doi.org/10.1083/jcb.137.6.1321.
Endow SA, Komma DJ. Spindle dynamics during meiosis in Drosophila oocytes. J Cell Biol. 1997 Jun 16;137(6):1321–36.
Endow, S. A., and D. J. Komma. “Spindle dynamics during meiosis in Drosophila oocytes.J Cell Biol, vol. 137, no. 6, June 1997, pp. 1321–36. Pubmed, doi:10.1083/jcb.137.6.1321.
Endow SA, Komma DJ. Spindle dynamics during meiosis in Drosophila oocytes. J Cell Biol. 1997 Jun 16;137(6):1321–1336.

Published In

J Cell Biol

DOI

ISSN

0021-9525

Publication Date

June 16, 1997

Volume

137

Issue

6

Start / End Page

1321 / 1336

Location

United States

Related Subject Headings

  • Transgenes
  • Spindle Apparatus
  • Recombinant Fusion Proteins
  • Oocytes
  • Mutation
  • Microtubule Proteins
  • Meiosis
  • Luminescent Proteins
  • Kinesins
  • Green Fluorescent Proteins